Establishment of a rat model of cervical syndrome with kidney deficiency

Jian Chun Jiang, Chen Guang Li, Qian Qian Liang, Qin Bian, Quan Zhou, Xue Jun Cui, Min Huang, Qing Gao Liu, Sheng Lu, Chong Jian Zhou, Qi Shi, Yong Jun Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To establish a rat model of cervical syndrome (CS) with kidney deficiency. Methods: A group of 30 three-month-old female Sprague-Dawley rats were randomly divided into normal control group, CS group and CS with kidney deficiency group (combined group), with 10 rats in each group. Rats in the normal control group received no treatment, rats in the CS group underwent only resection of cervical muscles and ligaments as unbalanced dynamic and static animal model, and rats in combined group underwent resection of both cervical muscles and ovaries as kidney deficiency model. Serum and cervical intervertebral discs were collected. Kidney deficiency was determined by observing the morphologic changes of uterus and appendages, detecting the weight of uterus and appendages and the content of serum estradiol (E2). The degeneration of intervertebral discs was determined by detecting the histopathology, the expressions of type II collagen and type X collagen proteins, and the expressions of aggrecan-1 (Agc1), type II procollagen gene (Col2a1), matrix metalloproteinase-13 (MMP-13) and tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNAs. Results: Compared with those in the normal control group and CS group, the rats in the combined group were noted with the uterus atrophied, the caliber of oviduct thinned, the weight of uterus and appendages diminished obviously, the content of serum E2 decreased, cervical intervertebral disc degenerated more seriously, type II collagen protein expression decreased, type X collagen protein expression increased, Agc1 and Col2a1 mRNA expressions in intervertebral disc decreased, and the MMP-13 mRNA expression increased. Conclusion: The rat model of CS with kidney deficiency is established. Kidney deficiency can aggravate cervical intervertebral disc degeneration.

Original languageEnglish (US)
Pages (from-to)1034-1039
Number of pages6
JournalJournal of Chinese Integrative Medicine
Volume6
Issue number10
DOIs
StatePublished - Oct 15 2008
Externally publishedYes

Keywords

  • Animal
  • Cervical syndrome
  • Combining disease and syndrome
  • Disease model
  • Kidney deficiency
  • Rats

ASJC Scopus subject areas

  • Complementary and alternative medicine

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