TY - JOUR
T1 - Establishment and characterization of an HTLV-I cell line from a Taiwanese patient with HTLV-I-associated myelopathy
AU - Yang, Ya Chien
AU - Hung, Tsu pei
AU - Wang, Chiu Hwa
AU - Lin, Ming Tseh
AU - Hsu, Tsuey Ying
AU - Chen, Jen Yang
AU - Chen, Yin Cheng
AU - Yang, Czau Siung
N1 - Funding Information:
AcknowledgementsW e arc gratefuli t, [)t~ l_J Su and H [-Tlcf~, and Miss H C ('hen for their excellentt echnicala ssistancem lhe experimentsT his study was supporteob y a grant from theN ahonal Science Counc(iNl SC 82-(1412-B-0[I2-14~-M0T2a1J pel, FaJwan
PY - 1993/12/1
Y1 - 1993/12/1
N2 - We describe a Taiwanese woman with chronic progressive myelopathy, in whom Western blot analysis of the serum and cerebrospinal fluid (CSF) displayed positive reactions to human T-lymphotropic virus type I (HTLV-I) proteins, p19, p24, p28, p36, gp46 and p53. HTLV-I proviral genomes were detected in the peripheral blood mononuclear cells (PBMC) and CSF cells by nested polymerase chain reaction and Southern blot hybridization. HTLV-I was successfully isolated from PBMC stimulated with interleukin-2 (IL-2). The established cell line, named THAM-1, was an IL-2-independent T-cell line with CD2+, CD3+, CD4+, CD25+ and HLA-DR+. Retrovirus particles with type C morphology were observed in the THAM-1 cells by electron microscopy, and HTLV-I-related antigens were also demonstrated by immunocytochemical staining and Western blot assay. Southern blot analysis revealed that HTLV-I proviral genomes were integrated into the THAM-1 cellular DNA. In Northern blot analysis, two extra-species of RNA were detected in addition to three typical viral transcripts. For the first time, an HTLV-I-producing T cell line was established from a patient with HTLV-I-associated myelopathy in Taiwan, an HTLV-I non-endemic area.
AB - We describe a Taiwanese woman with chronic progressive myelopathy, in whom Western blot analysis of the serum and cerebrospinal fluid (CSF) displayed positive reactions to human T-lymphotropic virus type I (HTLV-I) proteins, p19, p24, p28, p36, gp46 and p53. HTLV-I proviral genomes were detected in the peripheral blood mononuclear cells (PBMC) and CSF cells by nested polymerase chain reaction and Southern blot hybridization. HTLV-I was successfully isolated from PBMC stimulated with interleukin-2 (IL-2). The established cell line, named THAM-1, was an IL-2-independent T-cell line with CD2+, CD3+, CD4+, CD25+ and HLA-DR+. Retrovirus particles with type C morphology were observed in the THAM-1 cells by electron microscopy, and HTLV-I-related antigens were also demonstrated by immunocytochemical staining and Western blot assay. Southern blot analysis revealed that HTLV-I proviral genomes were integrated into the THAM-1 cellular DNA. In Northern blot analysis, two extra-species of RNA were detected in addition to three typical viral transcripts. For the first time, an HTLV-I-producing T cell line was established from a patient with HTLV-I-associated myelopathy in Taiwan, an HTLV-I non-endemic area.
KW - HTLV-I
KW - HTLV-I cell line
KW - HTLV-I-associated myelopathy
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U2 - 10.1016/0022-510X(93)90023-R
DO - 10.1016/0022-510X(93)90023-R
M3 - Article
C2 - 7904619
AN - SCOPUS:0027740970
SN - 0022-510X
VL - 120
SP - 46
EP - 53
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
IS - 1
ER -