Establishing drug resistance in microorganisms by mass spectrometry

Plamen A. Demirev, Nathan S. Hagan, Miquel D. Antoine, Jeffrey S. Lin, Andrew B. Feldman

Research output: Contribution to journalReview article

Abstract

A rapid method to determine drug resistance in bacteria based on mass spectrometry is presented. In it, a mass spectrum of an intact microorganism grown in drug-containing stable isotope-labeled media is compared with a mass spectrum of the intact microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting characteristic mass shifts of one or more microorganism biomarkers using bioinformatics algorithms. Observing such characteristic mass shifts indicates that the microorganism is viable even in the presence of the drug, thus incorporating the isotopic label into characteristic biomarker molecules. The performance of the method is illustrated on the example of intact E. coli, grown in control (unlabeled) and 13C-labeled media, and analyzed by MALDI TOF MS. Algorithms for data analysis are presented as well. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)1194-1201
Number of pages8
JournalJournal of the American Society for Mass Spectrometry
Volume24
Issue number8
DOIs
StatePublished - Aug 2013

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Drug Resistance
Microorganisms
Mass spectrometry
Mass Spectrometry
Biomarkers
Pharmaceutical Preparations
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Computational Biology
Isotopes
Escherichia coli
Bacteria
Bioinformatics
Labels
Molecules

Keywords

  • Algorithm
  • Antibiotic resistance
  • Bacteria
  • Biomarker
  • Isotope labeling
  • MALDI

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

Establishing drug resistance in microorganisms by mass spectrometry. / Demirev, Plamen A.; Hagan, Nathan S.; Antoine, Miquel D.; Lin, Jeffrey S.; Feldman, Andrew B.

In: Journal of the American Society for Mass Spectrometry, Vol. 24, No. 8, 08.2013, p. 1194-1201.

Research output: Contribution to journalReview article

Demirev, Plamen A. ; Hagan, Nathan S. ; Antoine, Miquel D. ; Lin, Jeffrey S. ; Feldman, Andrew B. / Establishing drug resistance in microorganisms by mass spectrometry. In: Journal of the American Society for Mass Spectrometry. 2013 ; Vol. 24, No. 8. pp. 1194-1201.
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