Essential role for a heterogeneous nuclear ribonucleoprotein (hnRNP) in oogenesis: hrp40 is absent from the germ line in the dorsoventral mutant squid

Erika Matunis, Richard Kelley, Gideon Dreyfuss

Research output: Contribution to journalArticle

Abstract

The Drosophila melanogaster hrp40 proteins are abundant nuclear pre-mRNA- binding proteins that are similar to the heterogeneous nuclear ribonucleoprotein (hnRNP) A/B proteins of vertebrates. Recently, hrp40 has been shown to be encoded by the squid gene, which is required for dorsoventral axis formation during oogenesis. Eggs and embryos from homozygous squid mothers are severely dorsalized, and complete deletion of the squid gene results in lethality. Here we have examined the expression and localization of hrp40 in wild-type and squid1 mutant ovaries. Using a monoclonal antibody specific for hrp40, the same isoforms of hrp40 are detected in both wild-type and squid1 ovaries, but the amount of hrp40 is reduced in squid1 ovaries. Furthermore, immunolocalization of hrp40 in wild- type egg chambers shows that hrp40 is present in the nurse cells, oocyte, and follicle cells. In contrast, in squid1 mutant egg chambers, hrp40 is absent from the germ-line-derived nurse cells and oocyte, but it is detected in the somatic follicle cells. The absence of hrp40 from the germ-line-derived cells of developing egg chambers is likely to lead to the striking dorsalized phenotype of squid1 eggs. In addition, dramatic stage-specific changes in the cellular localization of hrp40 are seen; the protein found in the nurse cell nuclei during early stages of oogenesis migrates to the cytoplasm at later stages. These findings reveal dynamic patterns of expression and localization of hnRNP proteins during development and provide evidence for an essential role for hnRNP proteins.

Original languageEnglish (US)
Pages (from-to)2781-2784
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number7
StatePublished - Mar 29 1994
Externally publishedYes

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Heterogeneous-Nuclear Ribonucleoproteins
Decapodiformes
Oogenesis
Germ Cells
Ovum
Ovary
Nurses
Nuclear Proteins
Eggs
Oocytes
Drosophila Proteins
RNA Precursors
Gene Deletion
Cell Nucleus
Vertebrates
Carrier Proteins
Protein Isoforms
Cytoplasm
Embryonic Structures
Monoclonal Antibodies

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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title = "Essential role for a heterogeneous nuclear ribonucleoprotein (hnRNP) in oogenesis: hrp40 is absent from the germ line in the dorsoventral mutant squid",
abstract = "The Drosophila melanogaster hrp40 proteins are abundant nuclear pre-mRNA- binding proteins that are similar to the heterogeneous nuclear ribonucleoprotein (hnRNP) A/B proteins of vertebrates. Recently, hrp40 has been shown to be encoded by the squid gene, which is required for dorsoventral axis formation during oogenesis. Eggs and embryos from homozygous squid mothers are severely dorsalized, and complete deletion of the squid gene results in lethality. Here we have examined the expression and localization of hrp40 in wild-type and squid1 mutant ovaries. Using a monoclonal antibody specific for hrp40, the same isoforms of hrp40 are detected in both wild-type and squid1 ovaries, but the amount of hrp40 is reduced in squid1 ovaries. Furthermore, immunolocalization of hrp40 in wild- type egg chambers shows that hrp40 is present in the nurse cells, oocyte, and follicle cells. In contrast, in squid1 mutant egg chambers, hrp40 is absent from the germ-line-derived nurse cells and oocyte, but it is detected in the somatic follicle cells. The absence of hrp40 from the germ-line-derived cells of developing egg chambers is likely to lead to the striking dorsalized phenotype of squid1 eggs. In addition, dramatic stage-specific changes in the cellular localization of hrp40 are seen; the protein found in the nurse cell nuclei during early stages of oogenesis migrates to the cytoplasm at later stages. These findings reveal dynamic patterns of expression and localization of hnRNP proteins during development and provide evidence for an essential role for hnRNP proteins.",
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AU - Dreyfuss, Gideon

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N2 - The Drosophila melanogaster hrp40 proteins are abundant nuclear pre-mRNA- binding proteins that are similar to the heterogeneous nuclear ribonucleoprotein (hnRNP) A/B proteins of vertebrates. Recently, hrp40 has been shown to be encoded by the squid gene, which is required for dorsoventral axis formation during oogenesis. Eggs and embryos from homozygous squid mothers are severely dorsalized, and complete deletion of the squid gene results in lethality. Here we have examined the expression and localization of hrp40 in wild-type and squid1 mutant ovaries. Using a monoclonal antibody specific for hrp40, the same isoforms of hrp40 are detected in both wild-type and squid1 ovaries, but the amount of hrp40 is reduced in squid1 ovaries. Furthermore, immunolocalization of hrp40 in wild- type egg chambers shows that hrp40 is present in the nurse cells, oocyte, and follicle cells. In contrast, in squid1 mutant egg chambers, hrp40 is absent from the germ-line-derived nurse cells and oocyte, but it is detected in the somatic follicle cells. The absence of hrp40 from the germ-line-derived cells of developing egg chambers is likely to lead to the striking dorsalized phenotype of squid1 eggs. In addition, dramatic stage-specific changes in the cellular localization of hrp40 are seen; the protein found in the nurse cell nuclei during early stages of oogenesis migrates to the cytoplasm at later stages. These findings reveal dynamic patterns of expression and localization of hnRNP proteins during development and provide evidence for an essential role for hnRNP proteins.

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