Essential Ca2+-binding motif for Ca2+-sensitive inactivation of L-type Ca2+ channels

Marita De Leon, Yan Wang, Lisa Jones, Edward Perez-Reyes, Xiangyang Wei, Tuck Wah Soong, Terry P. Snutch, David T. Yue

Research output: Contribution to journalArticle

Abstract

Intracellular calcium (Ca2+) inhibits the opening of L-type (α1C) Ca2+ channels, providing physiological control of Ca2+ entry into a wide variety of cells. A structural determinant of this Ca2+-sensitive inactivation was revealed by chimeric Ca2+ channels derived from parental α1C and α1E channels, the latter of which is a neuronal channel lacking Ca2+ inactivation. A consensus Ca2+-binding motif (an EF hand), located on the α1C subunit, was required for Ca2+ inactivation. Donation of the α1C EF-hand region to the α1E channel conferred the Ca2+-inactivating phenotype. These results strongly suggest that Ca2+ binding to the α1C subunit initiates Ca2+ inactivation.

Original languageEnglish (US)
Pages (from-to)1502-1506
Number of pages5
JournalScience
Volume270
Issue number5241
StatePublished - Dec 1 1995

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EF Hand Motifs
Calcium
Phenotype

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De Leon, M., Wang, Y., Jones, L., Perez-Reyes, E., Wei, X., Soong, T. W., ... Yue, D. T. (1995). Essential Ca2+-binding motif for Ca2+-sensitive inactivation of L-type Ca2+ channels. Science, 270(5241), 1502-1506.

Essential Ca2+-binding motif for Ca2+-sensitive inactivation of L-type Ca2+ channels. / De Leon, Marita; Wang, Yan; Jones, Lisa; Perez-Reyes, Edward; Wei, Xiangyang; Soong, Tuck Wah; Snutch, Terry P.; Yue, David T.

In: Science, Vol. 270, No. 5241, 01.12.1995, p. 1502-1506.

Research output: Contribution to journalArticle

De Leon, M, Wang, Y, Jones, L, Perez-Reyes, E, Wei, X, Soong, TW, Snutch, TP & Yue, DT 1995, 'Essential Ca2+-binding motif for Ca2+-sensitive inactivation of L-type Ca2+ channels', Science, vol. 270, no. 5241, pp. 1502-1506.
De Leon M, Wang Y, Jones L, Perez-Reyes E, Wei X, Soong TW et al. Essential Ca2+-binding motif for Ca2+-sensitive inactivation of L-type Ca2+ channels. Science. 1995 Dec 1;270(5241):1502-1506.
De Leon, Marita ; Wang, Yan ; Jones, Lisa ; Perez-Reyes, Edward ; Wei, Xiangyang ; Soong, Tuck Wah ; Snutch, Terry P. ; Yue, David T. / Essential Ca2+-binding motif for Ca2+-sensitive inactivation of L-type Ca2+ channels. In: Science. 1995 ; Vol. 270, No. 5241. pp. 1502-1506.
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AU - Jones, Lisa

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AU - Wei, Xiangyang

AU - Soong, Tuck Wah

AU - Snutch, Terry P.

AU - Yue, David T.

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