TY - JOUR
T1 - ESR1 amplification is rare in breast cancer and is associated with high grade and high proliferation
T2 - A multiplex ligation-dependent probe amplification study
AU - Moelans, Cathy B.
AU - Monsuur, Hanneke N.
AU - De Pinth, Johannes H.
AU - Radersma, Remco D.
AU - De Weger, Roel A.
AU - Van Diest, Paul J.
PY - 2011/10
Y1 - 2011/10
N2 - Background Expression of estrogen receptor alpha (ERa) is predictive for endocrine therapy response and an important prognostic factor in breast cancer. Overexpression of ERa can be caused by estrogen receptor 1 (ESR1) gene amplification and was originally reported to be a frequent event associated with a significantly longer survival for ER-positive women treated with adjuvant tamoxifen monotherapy, which was however questioned by subsequent studies. Methods This study aimed to reanalyze the frequency of ESR1 amplification by multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridisation (FISH), and to assess clinicopathologic correlations. MLPA was performed in a group of 135 breast cancer patients, and gains/amplifications were subjected to FISH. Results True ESR1 amplification by MLPA was rare (2%) and only 6% more patients showed a modest gain of ESR1. All MLPA-detected ESR1 amplifications and nearly all ESR1 gains were also FISH amplified and gained, but not all FISH amplifications/gains were MLPA amplified/ gained, leading to an overall concordance of only 60% between both techniques. All 3 MLPA and FISH ESR1 amplified cases had high ERa expression, but there was no obvious correlation between ESR1 gain and ER status by IHC. ESR1 gains/amplifications were not associated with HER2 gain/amplification, but seemed to be associated with older age. Surprisingly, ESR1 gain/amplification was not associated with low grade as reported previously, but correlated with high grade and high proliferation. Furthermore, ESR1 gain/amplification by MLPA was not associated with nodal status or tumor size (pT status). Conclusions ESR1 amplification as detected by MLPA is rare in breast cancer, and seems to be associated with high ERa expression, high age, high grade and high proliferation. This study confirms previous studies that showed differences in the ESR1 amplification frequencies detected by different techniques.
AB - Background Expression of estrogen receptor alpha (ERa) is predictive for endocrine therapy response and an important prognostic factor in breast cancer. Overexpression of ERa can be caused by estrogen receptor 1 (ESR1) gene amplification and was originally reported to be a frequent event associated with a significantly longer survival for ER-positive women treated with adjuvant tamoxifen monotherapy, which was however questioned by subsequent studies. Methods This study aimed to reanalyze the frequency of ESR1 amplification by multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridisation (FISH), and to assess clinicopathologic correlations. MLPA was performed in a group of 135 breast cancer patients, and gains/amplifications were subjected to FISH. Results True ESR1 amplification by MLPA was rare (2%) and only 6% more patients showed a modest gain of ESR1. All MLPA-detected ESR1 amplifications and nearly all ESR1 gains were also FISH amplified and gained, but not all FISH amplifications/gains were MLPA amplified/ gained, leading to an overall concordance of only 60% between both techniques. All 3 MLPA and FISH ESR1 amplified cases had high ERa expression, but there was no obvious correlation between ESR1 gain and ER status by IHC. ESR1 gains/amplifications were not associated with HER2 gain/amplification, but seemed to be associated with older age. Surprisingly, ESR1 gain/amplification was not associated with low grade as reported previously, but correlated with high grade and high proliferation. Furthermore, ESR1 gain/amplification by MLPA was not associated with nodal status or tumor size (pT status). Conclusions ESR1 amplification as detected by MLPA is rare in breast cancer, and seems to be associated with high ERa expression, high age, high grade and high proliferation. This study confirms previous studies that showed differences in the ESR1 amplification frequencies detected by different techniques.
KW - Amplification
KW - Breast cancer
KW - ESR1
KW - MLPA
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U2 - 10.1007/s13402-011-0045-5
DO - 10.1007/s13402-011-0045-5
M3 - Article
C2 - 21541733
AN - SCOPUS:84856201532
VL - 34
SP - 489
EP - 494
JO - Cellular oncology (Dordrecht)
JF - Cellular oncology (Dordrecht)
SN - 2211-3428
IS - 5
ER -