Esophagin cDNA cloning and characterization: A tissue-specific member of the small proline-rich protein family that is not expressed in esophageal tumors

John M. Abraham, Suna Wang, Hiroyuki Suzuki, Hai Yan Jiang, Lynne S. Rosenblum-Vos, Jing Yin, Stephen Meltzer

Research output: Contribution to journalArticle

Abstract

Cancer may be understood as the net effect of differences in gene expression between normal and transformed cells. In a novel direct approach applying this principle, complete genes expressed at altered mRNA levels in malignant versus normal esophageal epithelium were identified and isolated from cDNA libraries. One clone was expressed in normal esophageal mucosae but absent in esophageal carcinomas. By in situ hybridization, Northern blotting, and immunohistochemistry, expression of this gene was restricted to normal esophageal mucosa; it is designated esophagin. Esophagin expression was greatest in the superficial, most mature layers of esophageal squamous mucosa and was restricted to this organ, being undetectable in other squamous epithelia. A genomic clone localized esophagin to chromosomal region 1q21- q22. The expressed protein contains multiple direct repeats of an 8-amino acid motif rich in proline, with significant homology to the cornifin, pig 20K, monkey MT5, and human small proline-rich genes sprl and sprll. Esophagin constitutes the newest and largest member of this small proline-rich gene family and is associated with differentiation and the benign phenotype of the human esophageal epithelial cell.

Original languageEnglish (US)
Pages (from-to)855-860
Number of pages6
JournalCell Growth and Differentiation
Volume7
Issue number7
StatePublished - 1996
Externally publishedYes

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Cornified Envelope Proline-Rich Proteins
Proline
Organism Cloning
Complementary DNA
Neoplasms
Proteins
Epithelium
Clone Cells
Genes
Gene Expression
Amino Acid Motifs
Nucleic Acid Repetitive Sequences
Gene Library
Northern Blotting
Haplorhini
In Situ Hybridization
Swine
Epithelial Cells
Immunohistochemistry
Carcinoma

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Esophagin cDNA cloning and characterization : A tissue-specific member of the small proline-rich protein family that is not expressed in esophageal tumors. / Abraham, John M.; Wang, Suna; Suzuki, Hiroyuki; Jiang, Hai Yan; Rosenblum-Vos, Lynne S.; Yin, Jing; Meltzer, Stephen.

In: Cell Growth and Differentiation, Vol. 7, No. 7, 1996, p. 855-860.

Research output: Contribution to journalArticle

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