Escherichia coli K1 RS218 interacts with human brain microvascular endothelial cells via type 1 fimbria bacteria in the fimbriated state

Ching Hao Teng, Mian Cai, Sooan Shin, Yi Xie, Kee Jun Kim, Ahmed Khan Naveed, Francescopaolo Di Cello, Kwang Sik Kim

Research output: Contribution to journalArticle

Abstract

Escherichia coli K1 is a major gram-negative organism causing neonatal meningitis. E. coli K1 binding to and invasion of human brain microvascular endothelial cells (HBMEC) are a prerequisite for E. coli penetration into the central nervous system in vivo. In the present study, we showed using DNA microarray analysis that E. coli K1 associated with HBMEC expressed significantly higher levels of the fim genes compared to nonassociated bacteria. We also showed that E. coli K1 binding to and invasion of HBMEC were significantly decreased with its fimH deletion mutant and type 1 fimbria locked-off mutant, while they were significantly increased with its type 1 fimbria locked-on mutant. E. coli K1 strains associated with HBMEC were predominantly type 1 fimbria phase-on (i.e., fimbriated) bacteria. Taken together, we showed for the first time that type 1 fimbriae play an important role in E. coli K1 binding to and invasion of HBMEC and that type 1 fimbria phase-on E. coli is the major population interacting with HBMEC.

Original languageEnglish (US)
Pages (from-to)2923-2931
Number of pages9
JournalInfection and Immunity
Volume73
Issue number5
DOIs
StatePublished - May 2005

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Endothelial Cells
Escherichia coli
Bacteria
Brain
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Meningitis
Central Nervous System
Population
Genes

ASJC Scopus subject areas

  • Immunology

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Escherichia coli K1 RS218 interacts with human brain microvascular endothelial cells via type 1 fimbria bacteria in the fimbriated state. / Teng, Ching Hao; Cai, Mian; Shin, Sooan; Xie, Yi; Kim, Kee Jun; Naveed, Ahmed Khan; Di Cello, Francescopaolo; Kim, Kwang Sik.

In: Infection and Immunity, Vol. 73, No. 5, 05.2005, p. 2923-2931.

Research output: Contribution to journalArticle

Teng, Ching Hao ; Cai, Mian ; Shin, Sooan ; Xie, Yi ; Kim, Kee Jun ; Naveed, Ahmed Khan ; Di Cello, Francescopaolo ; Kim, Kwang Sik. / Escherichia coli K1 RS218 interacts with human brain microvascular endothelial cells via type 1 fimbria bacteria in the fimbriated state. In: Infection and Immunity. 2005 ; Vol. 73, No. 5. pp. 2923-2931.
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AU - Kim, Kee Jun

AU - Naveed, Ahmed Khan

AU - Di Cello, Francescopaolo

AU - Kim, Kwang Sik

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