Escherichia coli genes involved in resistance to pyrazinoic acid, the active component of the tuberculosis drug pyrazinamide

Alain Schaller, Ming Guo, Oluwatosin Gisanrin, Ying Zhang

Research output: Contribution to journalArticle

Abstract

The natural resistance of Escherichia coli to pyrazinoic acid (POA), the active derivative of pyrazinamide, was investigated. The TolC mutant was found to be more susceptible to POA and other weak acids than the wild-type strain. Mutation in EmrB but not AcrB efflux protein slightly increased POA susceptibility. Two transposon mutants with increased susceptibility to POA were found to harbor mutations in acnA encoding aconitase-1 and ygiY encoding a putative two-component sensor protein. Complementation of the AcnA and YgiY mutants conferred resistance to POA, whereas the complemented TolC mutant became resistant to POA and other weak acids.

Original languageEnglish (US)
Pages (from-to)265-270
Number of pages6
JournalFEMS microbiology letters
Volume211
Issue number2
DOIs
StatePublished - Jun 4 2002

Keywords

  • Efflux
  • Escherichia coli
  • Pyrazinoic acid
  • TolC
  • Weak acid susceptibility

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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