Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA)

J. W. Chase, Bruce Rabin, J. B. Murphy, K. L. Stone, K. R. Williams

Research output: Contribution to journalArticle

Abstract

We have determined the sequence of the gene encoding the large subunit of Escherichia coli exonuclease VII (xseA) and the amino acid sequence of the protein it encodes. The coding region of the xseA gene is 1368 base pairs. The protein encoded by the gene contains 456 amino acids and has a calculated molecular weight of 51,823. The promoter for xseA is close to that for guaB, and these two genes are transcribed in opposite directions: xseA clockwise and guaB counterclockwise on the standard E. coli genetic map. The cloned xseA gene can be complement an xseA deletion mutant strain. In an xseA+ genetic background production of large quantities of the xseA gene product appeared to decrease the amount of exonuclease VII activity in cell extracts. In fact, no exonuclease VII activity at all could be detected following inducton of strains in which the xseA gene was under λpL regulation. These observations suggest that the proper ratio of the large and small exonuclease VII subunits must be maintained in order to produce active enzyme.

Original languageEnglish (US)
Pages (from-to)14929-14935
Number of pages7
JournalJournal of Biological Chemistry
Volume261
Issue number32
StatePublished - Dec 1 1986
Externally publishedYes

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Exonucleases
Gene encoding
Cloning
Escherichia coli
Organism Cloning
Genes
Amino Acids
Cell Extracts
Base Pairing
Amino Acid Sequence
Proteins
Molecular Weight
Molecular weight
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA). / Chase, J. W.; Rabin, Bruce; Murphy, J. B.; Stone, K. L.; Williams, K. R.

In: Journal of Biological Chemistry, Vol. 261, No. 32, 01.12.1986, p. 14929-14935.

Research output: Contribution to journalArticle

Chase, J. W. ; Rabin, Bruce ; Murphy, J. B. ; Stone, K. L. ; Williams, K. R. / Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA). In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 32. pp. 14929-14935.
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