TY - JOUR
T1 - Escherichia coli binding to and invasion of brain microvascular endothelial cells derived from humans and rats of different ages
AU - Stins, Monique F.
AU - Nemani, Prasadarao V.
AU - Wass, Carol
AU - Kim, Kwang Sik
PY - 1999
Y1 - 1999
N2 - Escherichia coli meningitis commonly occurs in the neonatal period, but the basis of this age dependency is unclear. We have previously identified two types of E. coli-brain microvascular endothelial cell (BMEC) interactions contributing to E. coli traversal of the blood-brain barrier (i.e., binding and invasion). The present study examined whether the age dependency of E. coli meningitis stemmed from differences in the capacities of neonatal and adult BMECs to interact with E. coli. BMECs were isolated from rats of different ages (10 days, 20 days and 3 months) as well as from humans of different ages (fetuses, 4- to 7-year-old children, and a 35-year-old adult, and 60- to 85-year-old geriatrics). The bindings of E. coli to young and old rat BMECs were similar. Also, the abilities of E. coli to invade BMECs were similar for BMECs derived from young and old rats and from human fetuses, children, adults, and geriatrics. These findings suggest that the predominance of E. coli meningitis in neonates is not likely due to greater binding and invasion capacities of newborn compared to adult BMECs.
AB - Escherichia coli meningitis commonly occurs in the neonatal period, but the basis of this age dependency is unclear. We have previously identified two types of E. coli-brain microvascular endothelial cell (BMEC) interactions contributing to E. coli traversal of the blood-brain barrier (i.e., binding and invasion). The present study examined whether the age dependency of E. coli meningitis stemmed from differences in the capacities of neonatal and adult BMECs to interact with E. coli. BMECs were isolated from rats of different ages (10 days, 20 days and 3 months) as well as from humans of different ages (fetuses, 4- to 7-year-old children, and a 35-year-old adult, and 60- to 85-year-old geriatrics). The bindings of E. coli to young and old rat BMECs were similar. Also, the abilities of E. coli to invade BMECs were similar for BMECs derived from young and old rats and from human fetuses, children, adults, and geriatrics. These findings suggest that the predominance of E. coli meningitis in neonates is not likely due to greater binding and invasion capacities of newborn compared to adult BMECs.
UR - http://www.scopus.com/inward/record.url?scp=0032858908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032858908&partnerID=8YFLogxK
U2 - 10.1128/iai.67.10.5522-5525.1999
DO - 10.1128/iai.67.10.5522-5525.1999
M3 - Article
C2 - 10496943
AN - SCOPUS:0032858908
SN - 0019-9567
VL - 67
SP - 5522
EP - 5525
JO - Infection and immunity
JF - Infection and immunity
IS - 10
ER -