Erythrosin B (USFD&C RED 3) inhibits calcium transport and atpase activity of muscle sarcoplasmic reticulum

Stephen J. Morris, Ellen K. Silbergeld, Robin R. Brown, Duncan H. Haynes

Research output: Contribution to journalArticle

Abstract

Erythrosin B (USFD&C RED 3) inhibits the transport of calcium ions into isolated rabbit muscle sarcoplasmic reticulum vesicles with an IC50 of ∼ 0.5 μM and inhibits the Ca2+Mg2+ ATPase activity with an IC50 of ∼ 1 μM. The dye also binds to this tissue with an apparent Kd of ∼ 300 nM. Other iodinated and brominated fluorescein analogs and blue dextran also inhibit ATPase activity and displace bound dye, suggesting that erythrosin may bind to a site near to but not identical with the nucleotide site. The dye should prove to be a useful probe for transport and ATPase activity.

Original languageEnglish (US)
Pages (from-to)1306-1311
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume104
Issue number4
DOIs
StatePublished - Feb 26 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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