Erythrosin B (USFD&C RED 3) inhibits the transport of calcium ions into isolated rabbit muscle sarcoplasmic reticulum vesicles with an IC50 of ∼ 0.5 μM and inhibits the Ca2+Mg2+ ATPase activity with an IC50 of ∼ 1 μM. The dye also binds to this tissue with an apparent Kd of ∼ 300 nM. Other iodinated and brominated fluorescein analogs and blue dextran also inhibit ATPase activity and displace bound dye, suggesting that erythrosin may bind to a site near to but not identical with the nucleotide site. The dye should prove to be a useful probe for transport and ATPase activity.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Feb 26 1982|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology