Erythrosin B inhibits dopamine transport in rat caudate synaptosomes

Jeffrey A. Lafferman, Ellen K. Silbergeld

Research output: Contribution to journalArticlepeer-review

Abstract

Erythrosin B is a member of a class of fluorescein dyes that are sug-gested to elicit hyperkinesis when ingested by susceptible children. We found that erythrosin B inhibits dopamine uptake in rat caudate synaptosomes "uncompetilively" in the 10- to 800-micromolar range. Half maximal inhibition of uptake occurred at 45 micromolar. Uncompetitive inhibition denotes a decrease in efficacy of the dopamine membrane transport mechanism with an increase in affinity of dopa-mine to the carrier. Erythrosin B also decreased nonsaturable binding of dopamine to the synaptosome membrane. The inhibitory action of erythrosin B on dopamine uptake is consistent with the hypothesis that erythrosin B can act as a central ex citatory agent able to induce hyperkinetic behavior.

Original languageEnglish (US)
Pages (from-to)410-412
Number of pages3
JournalScience
Volume205
Issue number4404
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Erythrosin B inhibits dopamine transport in rat caudate synaptosomes'. Together they form a unique fingerprint.

Cite this