Error-prone polyploid mitosis during normal Drosophila development

Donald T. Fox, Joseph G. Gall, Allan C. Spradling

Research output: Contribution to journalArticle

Abstract

Endopolyploidy arises during normal development in many species when cells undergo endocycles - variant cell cycles in which DNA replicates but daughter cells do not form. Normally, polyploid cells do not divide mitotically after initiating endocycles; hence, little is known about their mitotic competence. However, polyploid cells are found in many tumors, and the enhanced chromosomal instability of polyploid cells in culture suggests that such cells contribute to tumor aneuploidy. Here, we describe a novel polyploid Drosophila cell type that undergoes normal mitotic cycles as part of a remodeling process that forms the adult rectal papillae. Similar polyploid mitotic divisions, but not depolyploidizing divisions, were observed during adult ileum development in the mosquito Culex pipiens. Extended anaphases, chromosome bridges, and lagging chromosomes were frequent during these polyploid divisions, despite normal expression of cell cycle regulators. Our results show that the switch to endocycles during development is not irreversible, but argue that the polyploid mitotic cycle is inherently error-prone, and that polyploid mitoses may help destabilize the cancer genome.

Original languageEnglish (US)
Pages (from-to)2294-2302
Number of pages9
JournalGenes & development
Volume24
Issue number20
DOIs
Publication statusPublished - Oct 15 2010
Externally publishedYes

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Keywords

  • Chromosomal instability
  • Drosophila
  • Endocycle
  • Hindgut
  • Polyploidy

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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