there was an error that affected the phylogenetic analysis presented in Figure. Below is a corrected version of Figure, which includes sequences from fecal and urine samples obtained from chimpanzees in our study and other related parasites (with Genbank accession numbers). Full-length (250 ±1 nt) Illumina reads from fecal samples were paired by name and merged using Geneious R9 (Kearse et al.,). The resulting 25 unique sequences with TFR-primer sequences were retained. Sequences from urine were acquired using traditional cloning methods from a subset of urine samples (N = 16 samples) instead of Illumina due to trace contaminants among the Illumina reads. We used uclust (Edgar,) implemented in MacQIIME to consolidate sequences with >97% similarity into operational taxonomic units (OTUs) which resulted in three fecal OTUs and five urine OTUs. All sequences were aligned using Geneious alignment with default settings. Phylogenetic reconstruction was performed in Mega7 (Kumar, Stecher, & Tamura,) (Fig.). The centroid sequence from each OTU was submitted to Genbank (accession numbers KX017526-KX017533). 2 (Figure presented.) Similar to the original figure, the corrected figure provides additional support for fecal-oral transmission of the identified Trichomonadidae infections. The paper conclusions remain unchanged. The authors regret this error.
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Animal Science and Zoology