ERMs colocalize transiently with L1 during neocortical axon outgrowth

Cyrus David Mintz, Tracey C. Dickson, Mark L. Gripp, Stephen R J Salton, Deanna L. Benson

Research output: Contribution to journalArticle

Abstract

L1 is a member of the Ig superfamily of cell adhesion molecules (CAMs) that functions in many aspects of neuronal development including axonal outgrowth and neuronal migration. These functions require coordination between L1 and the actin cytoskeleton. Because CAMs and the cytoskeleton do not bind directly, membrane-cytoskeletal linkers (MCLs) such as ankyrin are thought to be crucial to their interactions, but data from a knockout mouse suggest that ankyrin is not necessary for the earliest events attributed to L1 function. Recent findings in hippocampal cell culture show that members of the ERM family of proteins (ezrin, radixin, and moesin) can also serve as MCLs between L1 and actin in neurons. Here, we demonstrate that ERM proteins are expressed in extending neuronal processes in the intermediate zone of the developing cortex, a region that is densely packed with migrating neurons and growing axons: ERMs and L1 are codistributed extensively over a transient time course that coincides with rapid axon growth and cortical expansion. This codistribution is strong at embryonic day 17 and 19 but diminishes by postnatal day 0, at which time ankyrin-L1 codistribution increases dramatically. These findings suggest that in the developing neocortex, ERMs are the predominant MCL for L1 during migration and axon extension, neither of which requires ankyrin function. Furthermore, these data suggest that there is a developmentally regulated switch in MCL function in the developing brain.

Original languageEnglish (US)
Pages (from-to)438-448
Number of pages11
JournalJournal of Comparative Neurology
Volume464
Issue number4
DOIs
StatePublished - Aug 2003
Externally publishedYes

Fingerprint

Ankyrins
Axons
Membranes
Cell Adhesion Molecules
Neurons
Neocortex
Cytoskeleton
Actin Cytoskeleton
Knockout Mice
Actins
Proteins
Cell Culture Techniques
Neuronal Outgrowth
Brain
Growth

Keywords

  • Ankyrin
  • Cell adhesion molecule
  • Ezrin
  • Moesin
  • Neuronal migration
  • Radixin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

ERMs colocalize transiently with L1 during neocortical axon outgrowth. / Mintz, Cyrus David; Dickson, Tracey C.; Gripp, Mark L.; Salton, Stephen R J; Benson, Deanna L.

In: Journal of Comparative Neurology, Vol. 464, No. 4, 08.2003, p. 438-448.

Research output: Contribution to journalArticle

Mintz, Cyrus David ; Dickson, Tracey C. ; Gripp, Mark L. ; Salton, Stephen R J ; Benson, Deanna L. / ERMs colocalize transiently with L1 during neocortical axon outgrowth. In: Journal of Comparative Neurology. 2003 ; Vol. 464, No. 4. pp. 438-448.
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