TY - JOUR
T1 - Eradication of Helicobacter spp. by using medicated diet in mice deficient in functional natural killer cells and complement factor D
AU - Martino-Cardona, Maria Del Carmen
AU - Beck, Sarah E.
AU - Brayton, Cory
AU - Watson, Julie
PY - 2010/5
Y1 - 2010/5
N2 - A commercial 4-drug diet has shown promise in eradicating Helicobacter spp. from rodents; however, its effectiveness in immunocompromised mice is unknown. This study evaluated the efficacy of this treatment in eradicating Helicobacter spp. from mice deficient in functional natural killer cells (Cd1-/-) or complement factor D (Df-/-). Cd1-/- mice naturally infected with H. hepaticus with or without H. rodentium were fed either control or medicated diet for 8 wk followed by 4 wk on control diet. Fecal samples were PCR-evaluated for Helicobacter spp. before mice began treatment and then every 2 wk thereafter for 12 wk. The same experimental design was repeated for eighteen 9- to 21-wk-old Df-/- mice naturally infected with H. bilis with or without H. rodentium. All Df-/- mice and 8- to 21-wk-old Cd1 -/- mice ceased shedding Helicobacter spp. after 2 wk of treatment and remained negative throughout the study. In contrast, the Cd1-/- mice that were 24 wk or older shed Helicobacter spp. for the first 8 wk but tested negative at 10 and 12 wk. All treated animals had enlarged ceca and gained less weight than control untreated mice, and 6 of 7 treated Cd1 -/- male mice developed mild portal fibrosis. These findings show that within 2 wk of treatment, the 4-drug diet eradicated H. hepaticus and H. rodentium from young Cd1-/- mice and H. bilis and H. rodentium from Df-/- mice, but eradication of established infections in Cd1 -/- mice required 8 wk of treatment.
AB - A commercial 4-drug diet has shown promise in eradicating Helicobacter spp. from rodents; however, its effectiveness in immunocompromised mice is unknown. This study evaluated the efficacy of this treatment in eradicating Helicobacter spp. from mice deficient in functional natural killer cells (Cd1-/-) or complement factor D (Df-/-). Cd1-/- mice naturally infected with H. hepaticus with or without H. rodentium were fed either control or medicated diet for 8 wk followed by 4 wk on control diet. Fecal samples were PCR-evaluated for Helicobacter spp. before mice began treatment and then every 2 wk thereafter for 12 wk. The same experimental design was repeated for eighteen 9- to 21-wk-old Df-/- mice naturally infected with H. bilis with or without H. rodentium. All Df-/- mice and 8- to 21-wk-old Cd1 -/- mice ceased shedding Helicobacter spp. after 2 wk of treatment and remained negative throughout the study. In contrast, the Cd1-/- mice that were 24 wk or older shed Helicobacter spp. for the first 8 wk but tested negative at 10 and 12 wk. All treated animals had enlarged ceca and gained less weight than control untreated mice, and 6 of 7 treated Cd1 -/- male mice developed mild portal fibrosis. These findings show that within 2 wk of treatment, the 4-drug diet eradicated H. hepaticus and H. rodentium from young Cd1-/- mice and H. bilis and H. rodentium from Df-/- mice, but eradication of established infections in Cd1 -/- mice required 8 wk of treatment.
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M3 - Article
C2 - 20587159
AN - SCOPUS:77953190704
SN - 1559-6109
VL - 49
SP - 294
EP - 299
JO - Journal of the American Association for Laboratory Animal Science
JF - Journal of the American Association for Laboratory Animal Science
IS - 3
ER -