Epstein-Barr viral load as a marker of lymphoma in AIDS patients

Hongxin Fan, Seong Cheol Kim, Chukwuemeka O. Chima, Bruce F. Israel, Kathleen M. Lawless, Phyllis A. Eagan, Sandra Elmore, Dominic T. Moore, Steven A. Schichman, Lode Swinnen, Margaret L. Gulley

Research output: Contribution to journalArticle

Abstract

Epstein-Barr virus (EBV) is implicated in the pathogenesis of acquired immunodeficiency syndrome (AIDS) lymphoma, and viral DNA is present within the malignant cells in about half of affected patients. We examined the extent to which EBV viral load is elevated in the plasma of AIDS lymphoma patients compared to AIDS patients with opportunistic infections. Sixty-one AIDS patients were studied including 35 with lymphoma (24 non-Hodgkin, six Hodgkin, and five brain lymphoma) and 26 with various opportunistic infections. In situ hybridization revealed EBV encoded RNA (EBER) expression in the malignant cells of 17/28 AIDS lymphomas (61%). In 232 serial plasma samples from 35 lymphoma patients and in 128 samples from AIDS controls, EBV viral load was assayed by quantitative-polymerase chain reaction (Q-PCR) using a Taq-Man probe targeting the BamH1Wsequence. EBV was detected in plasma from all 17 EBER-positive AIDS lymphoma patients, with viral loads ranging from 34 to 1,500,000 copies per ml (median 3,210). Viral load usually fell rapidly upon initiation of lymphoma therapy and remained undetectable except in two patients with persistent tumor. In 11 AIDS patients, whose lymphoma lacked EBER expression, and in 26 control patients without lymphoma, levels of EBV in plasma were usually low or undetectable (range 0-1,995 and 0-2,409, median 0 and 0, respectively). There was no association between EBV viral load and human immunodeficiency virus (HIV) load or CD4 count. In conclusion, EBV viral load shows promise as a tool to assist in diagnosis and management of EBV-related lymphoma patients.

Original languageEnglish (US)
Pages (from-to)59-69
Number of pages11
JournalJournal of Medical Virology
Volume75
Issue number1
DOIs
StatePublished - Jan 2005

Fingerprint

Viral Load
Human Herpesvirus 4
Lymphoma
Acquired Immunodeficiency Syndrome
Opportunistic Infections
RNA
Viral DNA
CD4 Lymphocyte Count
Hodgkin Disease
Non-Hodgkin's Lymphoma
In Situ Hybridization
HIV
Polymerase Chain Reaction

Keywords

  • AIDS
  • Epstein-Barr virus
  • Lymphoma
  • Tumor marker
  • Viral load

ASJC Scopus subject areas

  • Virology

Cite this

Fan, H., Kim, S. C., Chima, C. O., Israel, B. F., Lawless, K. M., Eagan, P. A., ... Gulley, M. L. (2005). Epstein-Barr viral load as a marker of lymphoma in AIDS patients. Journal of Medical Virology, 75(1), 59-69. https://doi.org/10.1002/jmv.20238

Epstein-Barr viral load as a marker of lymphoma in AIDS patients. / Fan, Hongxin; Kim, Seong Cheol; Chima, Chukwuemeka O.; Israel, Bruce F.; Lawless, Kathleen M.; Eagan, Phyllis A.; Elmore, Sandra; Moore, Dominic T.; Schichman, Steven A.; Swinnen, Lode; Gulley, Margaret L.

In: Journal of Medical Virology, Vol. 75, No. 1, 01.2005, p. 59-69.

Research output: Contribution to journalArticle

Fan, H, Kim, SC, Chima, CO, Israel, BF, Lawless, KM, Eagan, PA, Elmore, S, Moore, DT, Schichman, SA, Swinnen, L & Gulley, ML 2005, 'Epstein-Barr viral load as a marker of lymphoma in AIDS patients', Journal of Medical Virology, vol. 75, no. 1, pp. 59-69. https://doi.org/10.1002/jmv.20238
Fan H, Kim SC, Chima CO, Israel BF, Lawless KM, Eagan PA et al. Epstein-Barr viral load as a marker of lymphoma in AIDS patients. Journal of Medical Virology. 2005 Jan;75(1):59-69. https://doi.org/10.1002/jmv.20238
Fan, Hongxin ; Kim, Seong Cheol ; Chima, Chukwuemeka O. ; Israel, Bruce F. ; Lawless, Kathleen M. ; Eagan, Phyllis A. ; Elmore, Sandra ; Moore, Dominic T. ; Schichman, Steven A. ; Swinnen, Lode ; Gulley, Margaret L. / Epstein-Barr viral load as a marker of lymphoma in AIDS patients. In: Journal of Medical Virology. 2005 ; Vol. 75, No. 1. pp. 59-69.
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