Epstein-Barr-encoded RNA in situ hybridization: Diagnostic applications

Richard F. Ambinder; Risa B. Mann

doi:10.1016/0046-8177(94)90227-5

Epstein-Barr-encoded RNA in situ hybridization: Diagnostic applications

Richard F. Ambinder, Risa B. Mann

School of Medicine

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

Latent Epstein-Barr virus (EBV) infection is associated with a variety of malignancies and other diseases. Highly restricted viral antigen expression and low viral genome copy number in infected tissues impede conventional immunohistochemical or in situ hybridization approaches to the detection of virus in these tissues. In situ hybridization detection of two small but very abundant nuclear RNAs known as the EBERs serves as an alternative approach. The EBERs are stable over time and can be detected in all common fixatives. This technique facilitates characterization of the cellular locus of latent EBV infection in histologically complex tissues, such as Hodgkin's disease and angioimmunoblastic lymphadenopathy with dysproteinemia.

Original language	English (US)
Pages (from-to)	602-605
Number of pages	4
Journal	Human pathology
Volume	25
Issue number	6
DOIs	https://doi.org/10.1016/0046-8177(94)90227-5
State	Published - Jun 1994

Keywords

EBER
Epstein-Barr
Hodgkin's disease
in situ hybridization
nasopharyngeal carcinoma

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1016/0046-8177(94)90227-5

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title = "Epstein-Barr-encoded RNA in situ hybridization: Diagnostic applications",

abstract = "Latent Epstein-Barr virus (EBV) infection is associated with a variety of malignancies and other diseases. Highly restricted viral antigen expression and low viral genome copy number in infected tissues impede conventional immunohistochemical or in situ hybridization approaches to the detection of virus in these tissues. In situ hybridization detection of two small but very abundant nuclear RNAs known as the EBERs serves as an alternative approach. The EBERs are stable over time and can be detected in all common fixatives. This technique facilitates characterization of the cellular locus of latent EBV infection in histologically complex tissues, such as Hodgkin's disease and angioimmunoblastic lymphadenopathy with dysproteinemia.",

keywords = "EBER, Epstein-Barr, Hodgkin's disease, in situ hybridization, nasopharyngeal carcinoma",

author = "Ambinder, {Richard F.} and Mann, {Risa B.}",

note = "Funding Information: From the Departments of Oncology, Pathology, Pharmacology, and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD. Accepted for publication April 24, 1994. Supported by the American Cancer Society grant no. EDT-1OB to R.F.A. K&yw ords: Epstein-Barr, in situ hybridization, EBER, nasopharyngeal carcinoma, Hodgkin{\textquoteright}s disease. Address correspondence and reprint requests to Richard F. Am-binder, MD, PhD, 418 N Bond St, Baltimore, MD 21231. Copyright 0 1994 by W.B. Saunders Company 0046-8177/94/25060011$5.00/0",

year = "1994",

month = jun,

doi = "10.1016/0046-8177(94)90227-5",

language = "English (US)",

volume = "25",

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journal = "Human pathology",

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TY - JOUR

T1 - Epstein-Barr-encoded RNA in situ hybridization

T2 - Diagnostic applications

AU - Ambinder, Richard F.

AU - Mann, Risa B.

N1 - Funding Information: From the Departments of Oncology, Pathology, Pharmacology, and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD. Accepted for publication April 24, 1994. Supported by the American Cancer Society grant no. EDT-1OB to R.F.A. K&yw ords: Epstein-Barr, in situ hybridization, EBER, nasopharyngeal carcinoma, Hodgkin’s disease. Address correspondence and reprint requests to Richard F. Am-binder, MD, PhD, 418 N Bond St, Baltimore, MD 21231. Copyright 0 1994 by W.B. Saunders Company 0046-8177/94/25060011$5.00/0

PY - 1994/6

Y1 - 1994/6

N2 - Latent Epstein-Barr virus (EBV) infection is associated with a variety of malignancies and other diseases. Highly restricted viral antigen expression and low viral genome copy number in infected tissues impede conventional immunohistochemical or in situ hybridization approaches to the detection of virus in these tissues. In situ hybridization detection of two small but very abundant nuclear RNAs known as the EBERs serves as an alternative approach. The EBERs are stable over time and can be detected in all common fixatives. This technique facilitates characterization of the cellular locus of latent EBV infection in histologically complex tissues, such as Hodgkin's disease and angioimmunoblastic lymphadenopathy with dysproteinemia.

AB - Latent Epstein-Barr virus (EBV) infection is associated with a variety of malignancies and other diseases. Highly restricted viral antigen expression and low viral genome copy number in infected tissues impede conventional immunohistochemical or in situ hybridization approaches to the detection of virus in these tissues. In situ hybridization detection of two small but very abundant nuclear RNAs known as the EBERs serves as an alternative approach. The EBERs are stable over time and can be detected in all common fixatives. This technique facilitates characterization of the cellular locus of latent EBV infection in histologically complex tissues, such as Hodgkin's disease and angioimmunoblastic lymphadenopathy with dysproteinemia.

KW - EBER

KW - Epstein-Barr

KW - Hodgkin's disease

KW - in situ hybridization

KW - nasopharyngeal carcinoma

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AN - SCOPUS:0028362329

SN - 0046-8177

VL - 25

SP - 602

EP - 605

JO - Human pathology

JF - Human pathology

IS - 6

ER -

Epstein-Barr-encoded RNA in situ hybridization: Diagnostic applications

Abstract

Keywords

ASJC Scopus subject areas

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