EPR spectroscopy and imaging of oxygen: Applications to the gastrointestinal tract

Jay L. Zweier, Guanglong He, Alexandre Samouilov, Periannan Kuppusamy

Research output: Contribution to journalArticle

Abstract

EPR imaging has emerged as an important tool for noninvasive three-dimensional (3D) spatial mapping of free radicals in biological tissues. Spectral-spatial EPR imaging enables mapping of the spectral information at each spatial position, and, from the observed linewidth, the localized tissue oxygenation can be mapped. We report the application of EPR imaging techniques enabling 3D spatial and spectral-spatial EPR imaging of small animals. This instrumentation, along with the use of a biocompatible charcoal oximetry-probe suspension, enabled 3D spatial imaging of the gastrointestinal (GI) tract, along with mapping of oxygenation in living mice. By using this technique, the oxygen tension was mapped at different levels of the GI tract from the stomach to the rectum. The results clearly show the presence of a marked oxygen gradient from the proximal to the distal GI tract, which decreases after respiratory arrest. This technique for in vivo mapping of oxygenation is a promising method, enabling the noninvasive imaging of oxygen within the normal GI tract. This method should be useful in determining the alterations in oxygenation associated with disease.

Original languageEnglish (US)
Pages (from-to)123-131
Number of pages9
JournalAdvances in Experimental Medicine and Biology
Volume530
StatePublished - 2003
Externally publishedYes

Fingerprint

Paramagnetic resonance
Gastrointestinal Tract
Spectrum Analysis
Oxygenation
Spectroscopy
Oxygen
Imaging techniques
Oximetry
Charcoal
Rectum
Tissue
Free Radicals
Stomach
Suspensions
Linewidth
Animals

Keywords

  • Activated charcoal
  • EPRI
  • Free radical
  • Oximetry

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

EPR spectroscopy and imaging of oxygen : Applications to the gastrointestinal tract. / Zweier, Jay L.; He, Guanglong; Samouilov, Alexandre; Kuppusamy, Periannan.

In: Advances in Experimental Medicine and Biology, Vol. 530, 2003, p. 123-131.

Research output: Contribution to journalArticle

Zweier, Jay L. ; He, Guanglong ; Samouilov, Alexandre ; Kuppusamy, Periannan. / EPR spectroscopy and imaging of oxygen : Applications to the gastrointestinal tract. In: Advances in Experimental Medicine and Biology. 2003 ; Vol. 530. pp. 123-131.
@article{8062e9806a944142ab9c2359029c7a0d,
title = "EPR spectroscopy and imaging of oxygen: Applications to the gastrointestinal tract",
abstract = "EPR imaging has emerged as an important tool for noninvasive three-dimensional (3D) spatial mapping of free radicals in biological tissues. Spectral-spatial EPR imaging enables mapping of the spectral information at each spatial position, and, from the observed linewidth, the localized tissue oxygenation can be mapped. We report the application of EPR imaging techniques enabling 3D spatial and spectral-spatial EPR imaging of small animals. This instrumentation, along with the use of a biocompatible charcoal oximetry-probe suspension, enabled 3D spatial imaging of the gastrointestinal (GI) tract, along with mapping of oxygenation in living mice. By using this technique, the oxygen tension was mapped at different levels of the GI tract from the stomach to the rectum. The results clearly show the presence of a marked oxygen gradient from the proximal to the distal GI tract, which decreases after respiratory arrest. This technique for in vivo mapping of oxygenation is a promising method, enabling the noninvasive imaging of oxygen within the normal GI tract. This method should be useful in determining the alterations in oxygenation associated with disease.",
keywords = "Activated charcoal, EPRI, Free radical, Oximetry",
author = "Zweier, {Jay L.} and Guanglong He and Alexandre Samouilov and Periannan Kuppusamy",
year = "2003",
language = "English (US)",
volume = "530",
pages = "123--131",
journal = "Advances in Experimental Medicine and Biology",
issn = "0065-2598",
publisher = "Springer New York",

}

TY - JOUR

T1 - EPR spectroscopy and imaging of oxygen

T2 - Applications to the gastrointestinal tract

AU - Zweier, Jay L.

AU - He, Guanglong

AU - Samouilov, Alexandre

AU - Kuppusamy, Periannan

PY - 2003

Y1 - 2003

N2 - EPR imaging has emerged as an important tool for noninvasive three-dimensional (3D) spatial mapping of free radicals in biological tissues. Spectral-spatial EPR imaging enables mapping of the spectral information at each spatial position, and, from the observed linewidth, the localized tissue oxygenation can be mapped. We report the application of EPR imaging techniques enabling 3D spatial and spectral-spatial EPR imaging of small animals. This instrumentation, along with the use of a biocompatible charcoal oximetry-probe suspension, enabled 3D spatial imaging of the gastrointestinal (GI) tract, along with mapping of oxygenation in living mice. By using this technique, the oxygen tension was mapped at different levels of the GI tract from the stomach to the rectum. The results clearly show the presence of a marked oxygen gradient from the proximal to the distal GI tract, which decreases after respiratory arrest. This technique for in vivo mapping of oxygenation is a promising method, enabling the noninvasive imaging of oxygen within the normal GI tract. This method should be useful in determining the alterations in oxygenation associated with disease.

AB - EPR imaging has emerged as an important tool for noninvasive three-dimensional (3D) spatial mapping of free radicals in biological tissues. Spectral-spatial EPR imaging enables mapping of the spectral information at each spatial position, and, from the observed linewidth, the localized tissue oxygenation can be mapped. We report the application of EPR imaging techniques enabling 3D spatial and spectral-spatial EPR imaging of small animals. This instrumentation, along with the use of a biocompatible charcoal oximetry-probe suspension, enabled 3D spatial imaging of the gastrointestinal (GI) tract, along with mapping of oxygenation in living mice. By using this technique, the oxygen tension was mapped at different levels of the GI tract from the stomach to the rectum. The results clearly show the presence of a marked oxygen gradient from the proximal to the distal GI tract, which decreases after respiratory arrest. This technique for in vivo mapping of oxygenation is a promising method, enabling the noninvasive imaging of oxygen within the normal GI tract. This method should be useful in determining the alterations in oxygenation associated with disease.

KW - Activated charcoal

KW - EPRI

KW - Free radical

KW - Oximetry

UR - http://www.scopus.com/inward/record.url?scp=0242330652&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242330652&partnerID=8YFLogxK

M3 - Article

C2 - 14562710

AN - SCOPUS:0242330652

VL - 530

SP - 123

EP - 131

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

SN - 0065-2598

ER -