EPR oxygen mapping (EPROM) of engineered cartilage grown in a hollow-fiber bioreactor

Scott J. Ellis, M. Velayutham, S. Sendhil Velan, Erik F. Petersen, Jay L. Zweier, Periannan Kuppusamy, Richard G S Spencer

Research output: Contribution to journalArticle

Abstract

A novel electron paramagnetic resonance (EPR)-based oxygen mapping procedure (EPROM) is applied to cartilage grown in a single-, hollow-fiber bioreactor (HFBR) system. Chondrocytes harvested from the sterna of 17-day-old chick embryos were inoculated into an HFBR and produced hyaline cartilage over a period of 4 weeks. Tissue oxygen maps were generated according to the EPROM technique (Velan et al., Magn Reson Med 2000;43:804-809) by making use of the line-broadening effects of oxygen on the signal generated from nitroxide spin probes. In addition, the effect on oxygen consumption of the addition of cyanide to the tissue was investigated. Cyanide is a potent inhibitor of oxidative phosphorylation, and accordingly, given the constant provision of oxygen to the tissue, it would be expected to increase oxygen levels within the HFBR. The EPROM measurements showed a significant increase in oxygen concentration in the cartilage after the addition of cyanide. In contrast to other methods for studying oxygen in cartilage, EPROM can provide direct, noninvasive visualization of local concentrations in three dimensions.

Original languageEnglish (US)
Pages (from-to)819-826
Number of pages8
JournalMagnetic Resonance in Medicine
Volume46
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Electron Spin Resonance Spectroscopy
Bioreactors
Cartilage
Oxygen
Cyanides
Hyaline Cartilage
Sternum
Oxidative Phosphorylation
Chick Embryo
Chondrocytes
Oxygen Consumption

Keywords

  • Cartilage
  • Chondrocytes
  • EPR
  • Oximetry
  • Oxygen mapping

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Ellis, S. J., Velayutham, M., Sendhil Velan, S., Petersen, E. F., Zweier, J. L., Kuppusamy, P., & Spencer, R. G. S. (2001). EPR oxygen mapping (EPROM) of engineered cartilage grown in a hollow-fiber bioreactor. Magnetic Resonance in Medicine, 46(4), 819-826. https://doi.org/10.1002/mrm.1262

EPR oxygen mapping (EPROM) of engineered cartilage grown in a hollow-fiber bioreactor. / Ellis, Scott J.; Velayutham, M.; Sendhil Velan, S.; Petersen, Erik F.; Zweier, Jay L.; Kuppusamy, Periannan; Spencer, Richard G S.

In: Magnetic Resonance in Medicine, Vol. 46, No. 4, 2001, p. 819-826.

Research output: Contribution to journalArticle

Ellis, SJ, Velayutham, M, Sendhil Velan, S, Petersen, EF, Zweier, JL, Kuppusamy, P & Spencer, RGS 2001, 'EPR oxygen mapping (EPROM) of engineered cartilage grown in a hollow-fiber bioreactor', Magnetic Resonance in Medicine, vol. 46, no. 4, pp. 819-826. https://doi.org/10.1002/mrm.1262
Ellis SJ, Velayutham M, Sendhil Velan S, Petersen EF, Zweier JL, Kuppusamy P et al. EPR oxygen mapping (EPROM) of engineered cartilage grown in a hollow-fiber bioreactor. Magnetic Resonance in Medicine. 2001;46(4):819-826. https://doi.org/10.1002/mrm.1262
Ellis, Scott J. ; Velayutham, M. ; Sendhil Velan, S. ; Petersen, Erik F. ; Zweier, Jay L. ; Kuppusamy, Periannan ; Spencer, Richard G S. / EPR oxygen mapping (EPROM) of engineered cartilage grown in a hollow-fiber bioreactor. In: Magnetic Resonance in Medicine. 2001 ; Vol. 46, No. 4. pp. 819-826.
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