Epitope-specific immune responses to rotavirus vaccination

Robert D. Shaw, Karen J. Fong, Genevieve A. Losonsky, Myron M. Levine, Yvonne Maldonado, Robert Yolken, Jorge Flores, Albert Z. Kapikian, Phuoc T. Vo, Harry B. Greenberg

Research output: Contribution to journalArticle

Abstract

Rotavirus gastroenteritis is a leading cause of infant mortality in developing countries and an important cause of morbidity in children under 2 yr of age in the United States. Vaccine programs have evaluated animal rotavirus strains that are attenuated in humans but antigenically similar to some human strains. Whether a single vaccine strain can elicit protective immunity in humans to rotaviruses of the same or different serotypes is an important question in determining vaccine efficacy. We used characterized serotype-specific monoclonal antibodies directed at VP7 in a competitive solid-phase immunoassay to measure epitope-specific immune responses to serotypes 1, 2, and 3 in sera of children who received a candidate serotype-3 rotavirus vaccine. Antibodies to serotype 3 were detected in 72% of sera samples, and to serotype 1 and 2 in only 11% each. Also, a VP3-specific monoclonal antibody which neutralizes three serotypically distinct strains of rotavirus was used to detect the presence of similar antibodies in 56% of the test sera. This finding suggests a mechanism of heterotypic immunity.

Original languageEnglish (US)
Pages (from-to)941-950
Number of pages10
JournalGastroenterology
Volume93
Issue number5
DOIs
StatePublished - Nov 1987

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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    Shaw, R. D., Fong, K. J., Losonsky, G. A., Levine, M. M., Maldonado, Y., Yolken, R., Flores, J., Kapikian, A. Z., Vo, P. T., & Greenberg, H. B. (1987). Epitope-specific immune responses to rotavirus vaccination. Gastroenterology, 93(5), 941-950. https://doi.org/10.1016/0016-5085(87)90555-5