Epitope-Resolved Detection of Peanut-Specific IgE Antibodies by Surface Plasmon Resonance Imaging

Min Shen, Amit A. Joshi, Raghu Vannam, Chandra K. Dixit, Robert G. Hamilton, Challa V. Kumar, James F. Rusling, Mark W. Peczuh

Research output: Contribution to journalArticlepeer-review

Abstract

Peanut allergy can be life-threatening and is mediated by allergen-specific immunoglobulin E (IgE) antibodies. Investigation of IgE antibody binding to allergenic epitopes can identify specific interactions underlying the allergic response. Here, we report a surface plasmon resonance imaging (SPRi) immunoassay for differentiating IgE antibodies by epitope-resolved detection. IgE antibodies were first captured by magnetic beads bearing IgE ϵ-chain-specific antibodies and then introduced into an SPRi array immobilized with epitopes from the major peanut allergen glycoprotein Arachis hypogaea h2 (Ara h2). Differential epitope responses were achieved by establishing a binding environment that minimized cross-reactivity while maximizing analytical sensitivity. IgE antibody binding to each Ara h2 epitope was distinguished and quantified from patient serum samples (10 μL each) in a 45 min assay. Excellent correlation of Ara h2-specific IgE values was found between ImmunoCAP assays and the new SPRi method.

Original languageEnglish (US)
Pages (from-to)199-202
Number of pages4
JournalChemBioChem
Volume19
Issue number3
DOIs
StatePublished - Feb 2 2018

Keywords

  • immunoassays
  • immunoglobulin E
  • magnetic bead
  • peanut allergy
  • surface plasmon resonance (SPR) imaging

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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