Epithelial interleukin-11: Regulation by cytokines, respiratory syncytial virus, and retinoic acid

Jack A. Elias, Tao Zheng, Oskar Einarsson, Marie Landry, Terence Trow, Nancy Rebert, James Panuska

Research output: Contribution to journalArticle

Abstract

Interleukin-11 (IL-11) is a pleotropic cytokine with effects that overlap with IL-6. To determine if IL-11 is produced by epithelial cells, we determined whether human alveolar A549 cells and airway 9HTE cells produce IL-11. We also determined whether retinoic acid (RA) altered this IL-11 production. Unstimulated cells produced low levels of IL-11, while IL-1, transforming growth factor (TGF-β1), and respiratory syncytial virus (RSV) stimulated IL-11 protein production and mRNA accumulation in a time- and dose-dependent fashion. IL-1 and TGF-β1, also interacted in a synergistic, and presumedly transcriptional, fashion since they augmented A549 cell IL-11 protein production and mRNA accumulation without altering IL-11 mRNA half-life. In contrast, IL-4 only weakly stimulated, and IL-7, hepatocyte growth factor, and herpes simplex virus Type 2 did not stimulate, IL-11 production. RA did not alter the IL-11 production of unstimulated or RSV infected cells. It did, however, inhibit rIL-1-stimulated and synergistically augment TGF-β-stimulated IL-11 production. Thus, IL-1, TGF-β, and RSV stimulate epithelial-like cell IL-11 production, and RA regulates these inductive processes in a stimulus-specific fashion.

Original languageEnglish (US)
Pages (from-to)22261-22268
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number35
StatePublished - Sep 2 1994
Externally publishedYes

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Interleukin-11
Respiratory Syncytial Viruses
Tretinoin
Viruses
Cytokines
Interleukin-1
Messenger RNA
Epithelial Cells
Alveolar Epithelial Cells
Interleukin-7
Human Herpesvirus 2
Hepatocyte Growth Factor
Transforming Growth Factors
Interleukin-4
Half-Life
Interleukin-6

ASJC Scopus subject areas

  • Biochemistry

Cite this

Elias, J. A., Zheng, T., Einarsson, O., Landry, M., Trow, T., Rebert, N., & Panuska, J. (1994). Epithelial interleukin-11: Regulation by cytokines, respiratory syncytial virus, and retinoic acid. Journal of Biological Chemistry, 269(35), 22261-22268.

Epithelial interleukin-11 : Regulation by cytokines, respiratory syncytial virus, and retinoic acid. / Elias, Jack A.; Zheng, Tao; Einarsson, Oskar; Landry, Marie; Trow, Terence; Rebert, Nancy; Panuska, James.

In: Journal of Biological Chemistry, Vol. 269, No. 35, 02.09.1994, p. 22261-22268.

Research output: Contribution to journalArticle

Elias, JA, Zheng, T, Einarsson, O, Landry, M, Trow, T, Rebert, N & Panuska, J 1994, 'Epithelial interleukin-11: Regulation by cytokines, respiratory syncytial virus, and retinoic acid', Journal of Biological Chemistry, vol. 269, no. 35, pp. 22261-22268.
Elias JA, Zheng T, Einarsson O, Landry M, Trow T, Rebert N et al. Epithelial interleukin-11: Regulation by cytokines, respiratory syncytial virus, and retinoic acid. Journal of Biological Chemistry. 1994 Sep 2;269(35):22261-22268.
Elias, Jack A. ; Zheng, Tao ; Einarsson, Oskar ; Landry, Marie ; Trow, Terence ; Rebert, Nancy ; Panuska, James. / Epithelial interleukin-11 : Regulation by cytokines, respiratory syncytial virus, and retinoic acid. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 35. pp. 22261-22268.
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