Epithelial boost enhances antigen expression by vaccinia virus for the generation of potent CD8+ T cell-mediated antitumor immunity following DNA priming vaccination

Jin Qiu, Shiwen Peng, Ying Ma, Andrew Yang, Emily Farmer, Max A. Cheng, Richard B.S. Roden, T. C. Wu, Yung Nien Chang, Chien Fu Hung

Research output: Contribution to journalArticle

Abstract

While both pNGVL4a-Sig/E7(detox)/HSP70 DNA vaccine and TA-HPV recombinant vaccinia viral vector-based vaccines have elicited HPV-specific CD8+ T cell responses in HPV16/E7-expressing tumor models, and been used as prime-boost regimen to enhance HPV-specific immune responses in humans (NCT00788164), the optimal route of administration for TA-HPV remains unclear. In a preclinical model, we examined the immunogenicity of priming with intramuscular pNGVL4a-Sig/E7(detox)/HSP70 followed by TA-HPV boost through different administration routes. We observed that priming twice with a pNGVL4a-Sig/E7(detox)/HSP70 followed by a single TA-HPV immunization boost through skin scarification generated the strongest antigen-specific CD8+ T cell response in C57BL/6 mice. These data translate to tumor control and prolonged survival of treated mice. Our results provide rationale for future clinical testing of intramuscular pNGVL4a-Sig/E7(detox)/HSP70 DNA vaccine prime, TA-HPV vaccine skin scarification boost immunization regimen for the control of HPV-associated diseases.

Original languageEnglish (US)
Pages (from-to)205-215
Number of pages11
JournalVirology
Volume525
DOIs
StatePublished - Dec 2018

Keywords

  • Human papillomavirus
  • Pre-clinical model
  • TA-HPV
  • Therapeutic HPV vaccine
  • pNGVL4a-Sig/E7(detox)/HSP70

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Epithelial boost enhances antigen expression by vaccinia virus for the generation of potent CD8+ T cell-mediated antitumor immunity following DNA priming vaccination'. Together they form a unique fingerprint.

  • Cite this