Epigenomic profiling of retinal progenitors reveals LHX2 is required for developmental regulation of open chromatin

Cristina Zibetti, Sheng Liu, Jun Wan, Jiang Qian, Seth Blackshaw

Research output: Contribution to journalArticlepeer-review

Abstract

Retinal neurogenesis occurs through partially overlapping temporal windows, driven by concerted actions of transcription factors which, in turn, may contribute to the establishment of divergent genetic programs in the developing retina by coordinating variations in chromatin landscapes. Here we comprehensively profile murine retinal progenitors by integrating next generation sequencing methods and interrogate changes in chromatin accessibility at embryonic and post-natal stages. An unbiased search for motifs in open chromatin regions identifies putative factors involved in the developmental progression of the epigenome in retinal progenitor cells. Among these factors, the transcription factor LHX2 exhibits a developmentally regulated cis-regulatory repertoire and stage-dependent motif instances. Using loss-of-function assays, we determine LHX2 coordinates variations in chromatin accessibility, by competition for nucleosome occupancy and secondary regulation of candidate pioneer factors.

Original languageEnglish (US)
Article number142
JournalCommunications biology
Volume2
Issue number1
DOIs
StatePublished - Dec 1 2019

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint

Dive into the research topics of 'Epigenomic profiling of retinal progenitors reveals LHX2 is required for developmental regulation of open chromatin'. Together they form a unique fingerprint.

Cite this