Epigenetically altered miR-1247 functions as a tumor suppressor in pancreatic cancer

Joo Mi Yi, Eun-Jin, Hyun-Mi, Jin Han Bae, Keunsoo Kang, Nita Ahuja, Kwangmo Yang

Research output: Contribution to journalArticle

Abstract

Altered expression of microRNAs has been strongly implicated in human cancers, and growing evidence is emerging that a number of miRNAs are downregulated in cancer associated with CpG island hypermethylation. Although pancreatic cancer is one of the most malignant human cancers, the roles of miRNAs underlying the tumorigenesis of pancreatic cancer are still poorly understood. In the present study, we explored the molecular functional role of microRNA-1247 as tumor suppressor associated with epigenetic alteration in pancreatic cancer. CpG islands methylation of miR-1247 is frequently observed in various pancreatic cancer cell lines and in primary pancreatic tumors, but not in normal pancreatic tissue. Ectopic expression of miR-1247 in five pancreatic cancer cell lines results in suppressing of cell growth, proliferation, migration, and invasion in vitro and tumorigenicity of pancreatic cancer cells in vivo. Interestingly, we found one putative target gene of miR-1247, regulator of chromosome condensation 2 (RCC2), harbored miR-1247 target sequences in the 3' UTR of its mRNA. In functional studies in vitro to understand the interaction between miR-1247 and RCC2, decreasing of RCC2 gene expression by miR-1247 was observed by immunoblotting and immunohistochemistry at both mRNA and protein levels. Moreover, luciferase reporter assay confirmed that RCC2 was a direct target of miR-1247. Taken together, our data suggest that CpG island hypermethylation of miR-1247 is responsible for its downregulation in pancreatic cancer, and ectopic expression of miR-1247 functions as a potential tumor suppressor targeting RCC2 in pancreatic cancer cells.

Original languageEnglish (US)
Pages (from-to)26600-26612
Number of pages13
JournalOncotarget
Volume8
Issue number16
DOIs
StatePublished - 2017

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Pancreatic Neoplasms
Chromosomes, Human, Pair 2
MicroRNAs
CpG Islands
Neoplasms
Down-Regulation
Cell Line
Messenger RNA
3' Untranslated Regions
Luciferases
Immunoblotting
Epigenomics
Methylation
Carcinogenesis
Immunohistochemistry
Cell Proliferation
Gene Expression
Growth
Genes
Proteins

Keywords

  • Hypermethylation
  • microRNA
  • MiR-1247
  • Pancreatic cancer
  • Tumor suppressor

ASJC Scopus subject areas

  • Oncology

Cite this

Epigenetically altered miR-1247 functions as a tumor suppressor in pancreatic cancer. / Yi, Joo Mi; Eun-Jin; Hyun-Mi; Bae, Jin Han; Kang, Keunsoo; Ahuja, Nita; Yang, Kwangmo.

In: Oncotarget, Vol. 8, No. 16, 2017, p. 26600-26612.

Research output: Contribution to journalArticle

Yi, JM, Eun-Jin, Hyun-Mi, Bae, JH, Kang, K, Ahuja, N & Yang, K 2017, 'Epigenetically altered miR-1247 functions as a tumor suppressor in pancreatic cancer', Oncotarget, vol. 8, no. 16, pp. 26600-26612. https://doi.org/10.18632/oncotarget.15722
Yi, Joo Mi ; Eun-Jin ; Hyun-Mi ; Bae, Jin Han ; Kang, Keunsoo ; Ahuja, Nita ; Yang, Kwangmo. / Epigenetically altered miR-1247 functions as a tumor suppressor in pancreatic cancer. In: Oncotarget. 2017 ; Vol. 8, No. 16. pp. 26600-26612.
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