Epigenetic regulation of stem cell maintenance in the drosophila testis via the nucleosome-remodeling factor NURF

Christopher M. Cherry, Erika L. Matunis

Research output: Contribution to journalArticlepeer-review

Abstract

Regulation of stem cells depends on both tissue-specific transcriptional regulators and changes in chromatin organization, yet the coordination of these events in endogenous niches is poorly understood. In the Drosophila testis, local JAK-STAT signaling maintains germline and somatic stem cells (GSCs and cyst progenitor cells, or CPCs) in a single niche. Here we show that epigenetic regulation via the nucleosome-remodeling factor (NURF) complex ensures GSC and CPC maintenance by positively regulating JAK-STAT signaling, thereby preventing premature differentiation. Conversely, NURF is not required in early differentiating daughter cells of either lineage. Because three additional ATP-dependent chromatin remodelers (ACF, CHRAC, and dMi-2/NuRD) are dispensable for stem cell maintenance in the testis, epigenetic regulation of stem cells within this niche may rely primarily on NURF. Thus, local signals cooperate with specific chromatin-remodeling complexes in intact niches to coordinately regulate a common set of target genes to prevent premature stem cell differentiation.

Original languageEnglish (US)
Pages (from-to)557-567
Number of pages11
JournalCell stem cell
Volume6
Issue number6
DOIs
StatePublished - Jun 4 2010

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Cell Biology

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