Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors

Emily A. Bassett, Stacey Wood, Kevan J. Salimian, Sandya Ajith, Daniel R. Foltz, Ben E. Black

Research output: Contribution to journalArticlepeer-review

Abstract

The nearly ubiquitous presence of repetitive centromere DNA sequences across eukaryotic species is in paradoxical contrast to their apparent functional dispensability. Centromeric chromatin is spatially delineated into the kinetochore-forming array of centromere protein A (CENP-A)-containing nucleosomes and the inner centromeric heterochromatin that lacks CENP-A but recruits the aurora B kinase that is necessary for correcting erroneous attachments to the mitotic spindle. We found that the self-perpetuating network of CENPs at the foundation of the kinetochore is intact at a human neocentromere lacking repetitive α-satellite DNA. However, aurora B is inappropriately silenced as a consequence of the altered geometry of the neocentromere, thereby compromising the error correction mechanism. This suggests a model wherein the neocentromere represents a primordial inheritance locus that requires subsequent generation of a robust inner centromere compartment to enhance fidelity of chromosome transmission.

Original languageEnglish (US)
Pages (from-to)177-185
Number of pages9
JournalJournal of Cell Biology
Volume190
Issue number2
DOIs
StatePublished - Jul 26 2010
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors'. Together they form a unique fingerprint.

Cite this