Epigallocatechin-3-gallate reduces airway inflammation in mice through binding to proinflammatory chemokines and inhibiting inflammatory cell recruitment

Shulin Qin, John F. Alcorn, Jodi K. Craigo, Charis Tjoeng, Patrick Tarwater, Jay K. Kolls, Todd A. Reinhart

Research output: Contribution to journalArticle

Abstract

One major activity of chemokines is the recruitment of immune cells to sites of infection and inflammation. CD4+ Th1 cells play critical roles in host defense against pathogens and in the pathogenesis of many immune-mediated diseases. It was reported that epigallocatechin-3-gallate (EGCG) exhibits anti-inflammatory properties, but the mechanisms have not been completely defined. In this study, we found that EGCG markedly decreased recruitment of murine OVA-specific Th1 cells and other inflammatory cells into the airways in a Th1 adoptive-transfer mouse model. In vitro analysis revealed that EGCG inhibited CXCR3 ligand-driven chemotaxis of murine and human cells. Surface plasmon resonance studies revealed that EGCG bound directly to chemokines CXCL9, CXCL10, and CXCL11. These results indicated that one anti-inflammatory mechanism of EGCG is binding of proinflammatory chemokines and limiting their biological activities. These findings support further development of EGCG as a potent therapeutic for inflammatory diseases.

Original languageEnglish (US)
Pages (from-to)3693-3700
Number of pages8
JournalJournal of Immunology
Volume186
Issue number6
DOIs
StatePublished - Mar 15 2011
Externally publishedYes

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Chemokines
Inflammation
Th1 Cells
Chemokine CXCL11
Chemokine CXCL9
Anti-Inflammatory Agents
Chemokine CXCL10
Surface Plasmon Resonance
Adoptive Transfer
Immune System Diseases
Chemotaxis
epigallocatechin gallate
Ligands
Infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Epigallocatechin-3-gallate reduces airway inflammation in mice through binding to proinflammatory chemokines and inhibiting inflammatory cell recruitment. / Qin, Shulin; Alcorn, John F.; Craigo, Jodi K.; Tjoeng, Charis; Tarwater, Patrick; Kolls, Jay K.; Reinhart, Todd A.

In: Journal of Immunology, Vol. 186, No. 6, 15.03.2011, p. 3693-3700.

Research output: Contribution to journalArticle

Qin, Shulin ; Alcorn, John F. ; Craigo, Jodi K. ; Tjoeng, Charis ; Tarwater, Patrick ; Kolls, Jay K. ; Reinhart, Todd A. / Epigallocatechin-3-gallate reduces airway inflammation in mice through binding to proinflammatory chemokines and inhibiting inflammatory cell recruitment. In: Journal of Immunology. 2011 ; Vol. 186, No. 6. pp. 3693-3700.
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AU - Kolls, Jay K.

AU - Reinhart, Todd A.

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