Abstract
Insulin-like growth factor I (IGF-I) synergistically enhances epidermal growth factor (EGF) -stimulated proliferation of intestinal epithelial cells. A possible mechanism of this synergy is that EGF acts as a "competence" factor increasing the fraction of proliferating cells by promoting transition from G0 to G1, thus allowing IGF-I, a "progression" factor, to act as a proliferative agent on the cycling population. Consistent with this hypothesis would be temporally distinct actions wherein initial brief exposure to EGF would permit synergy, whereas pretreatment with IGF-I would not. Rat intestinal epithelial cells of the IEC-18 crypt cell line were serum-deprived, then treated with EGF (5×10-9 M), IGF-I (5×10-9 M), or insulin (2×10-6 M) for a 30-min pulse and then media containing EGF, IGF-I, insulin, or no factor was substituted for 48 hr. IGF-I and EGF each stimulated enterocyte proliferation; together they synergistically promoted cell growth. A brief pulse of IGF-I neither induced cell proliferation nor enhanced the EGF effect. Initial brief exposure to EGF, however, was equally efficacious as continuous exposure and allowed full synergy with IGF-I. Insulin at supraphysiologic levels acted similarly to IGF-I. Thus, EGF acted as a competence factor priming the cells for subsequent action by IGF-I. The cell kinetic parameters of these growth factors may be important to both physiologic and pathologic enterocyte growth regulation.
Original language | English (US) |
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Pages (from-to) | 2197-2201 |
Number of pages | 5 |
Journal | Digestive diseases and sciences |
Volume | 39 |
Issue number | 10 |
DOIs | |
State | Published - Oct 1994 |
Externally published | Yes |
Keywords
- cell cycle
- crypt cell
- enterocyte
- epidermal growth factor
- insulin-like growth factors
- intestine
- proliferation
ASJC Scopus subject areas
- Physiology
- Gastroenterology