Epidermal growth factor-mediated apoptosis of MDA-MB-468 human breast cancer cells

Deborah Kay Armstrong, Scott H. Kaufmann, Yvonne L. Ottaviano, Yuzo Furuya, Julie A. Buckley, John Tod Isaacs, Nancy E. Davidson

Research output: Contribution to journalArticle

Abstract

MDA-MB-468 human breast cancer cells lack estrogen receptors, overexpress epidermal growth factor (EGF) receptors, and are growth inhibited by EGF. We show that treatment of MDA-MB-468 cells with EGF leads to inhibition of cell proliferation, fragmentation of DNA into nucleosomal oligomers, and the development of apoptotic morphology. This treatment is associated with increased expression of c-myc, c-fos, jun family members, and transforming growth factor β1 mRNA and with partial proteolytic cleavage of poly(ADP- ribose) polymerase and lamin B. The observation that EGF can mediate apoptosis in EGF receptor-overexpressing cells has important implications for clinical efforts directed at the EGF receptor.

Original languageEnglish (US)
Pages (from-to)5280-5283
Number of pages4
JournalCancer Research
Volume54
Issue number20
StatePublished - Oct 15 1994

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Epidermal Growth Factor
Apoptosis
Breast Neoplasms
Epidermal Growth Factor Receptor
Lamin Type B
Poly(ADP-ribose) Polymerases
Transforming Growth Factors
DNA Fragmentation
Estrogen Receptors
Cell Proliferation
Messenger RNA
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Armstrong, D. K., Kaufmann, S. H., Ottaviano, Y. L., Furuya, Y., Buckley, J. A., Isaacs, J. T., & Davidson, N. E. (1994). Epidermal growth factor-mediated apoptosis of MDA-MB-468 human breast cancer cells. Cancer Research, 54(20), 5280-5283.

Epidermal growth factor-mediated apoptosis of MDA-MB-468 human breast cancer cells. / Armstrong, Deborah Kay; Kaufmann, Scott H.; Ottaviano, Yvonne L.; Furuya, Yuzo; Buckley, Julie A.; Isaacs, John Tod; Davidson, Nancy E.

In: Cancer Research, Vol. 54, No. 20, 15.10.1994, p. 5280-5283.

Research output: Contribution to journalArticle

Armstrong, DK, Kaufmann, SH, Ottaviano, YL, Furuya, Y, Buckley, JA, Isaacs, JT & Davidson, NE 1994, 'Epidermal growth factor-mediated apoptosis of MDA-MB-468 human breast cancer cells', Cancer Research, vol. 54, no. 20, pp. 5280-5283.
Armstrong DK, Kaufmann SH, Ottaviano YL, Furuya Y, Buckley JA, Isaacs JT et al. Epidermal growth factor-mediated apoptosis of MDA-MB-468 human breast cancer cells. Cancer Research. 1994 Oct 15;54(20):5280-5283.
Armstrong, Deborah Kay ; Kaufmann, Scott H. ; Ottaviano, Yvonne L. ; Furuya, Yuzo ; Buckley, Julie A. ; Isaacs, John Tod ; Davidson, Nancy E. / Epidermal growth factor-mediated apoptosis of MDA-MB-468 human breast cancer cells. In: Cancer Research. 1994 ; Vol. 54, No. 20. pp. 5280-5283.
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