Abstract
Differentiation of pluripotent embryonic stem (ES) cells is associated with expression of fate-specifying gene products. Coordinated development, however, must involve modifying factors that enable differentiation and growth to adjust in response to local microenvironmental determinants. We report here that the ephrin receptor, EphB4, known to be spatially restricted in expression and critical for organized vessel formation, modifies the rate and magnitude of ES cells acquiring genotypic and phenotypic characteristics of mesodermal tissues. Hemangioblast, blood cell, cardiomyocyte, and vascular differentiation was impaired in EphB4-/-ES cells in conjunction with decreased expression of mesoderm-associated, but not neuroectoderm-associated, genes. Therefore, EphB4 modulates the response to mesoderm induction signals. These data add differentiation kinetics to the known effects of ephrin receptors on mammalian cell migration and adhesion. We propose that modifying sensitivity to differentiation cues is a further means for ephrin receptors to contribute to tissue patterning and organization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 100-109 |
| Number of pages | 10 |
| Journal | Blood |
| Volume | 103 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Hematology
Cite this
Ephrin receptor, EphB4, regulates ES cell differentiation of primitive mammalian hemangioblasts, blood, cardiomyocytes, and blood vessels. / Wang, Zack Z.; Cohen, Kenneth; Shao, Ying; Mole, Pamela; Dombkowski, David; Scadden, David T.
In: Blood, Vol. 103, No. 1, 01.01.2004, p. 100-109.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Ephrin receptor, EphB4, regulates ES cell differentiation of primitive mammalian hemangioblasts, blood, cardiomyocytes, and blood vessels
AU - Wang, Zack Z.
AU - Cohen, Kenneth
AU - Shao, Ying
AU - Mole, Pamela
AU - Dombkowski, David
AU - Scadden, David T.
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Differentiation of pluripotent embryonic stem (ES) cells is associated with expression of fate-specifying gene products. Coordinated development, however, must involve modifying factors that enable differentiation and growth to adjust in response to local microenvironmental determinants. We report here that the ephrin receptor, EphB4, known to be spatially restricted in expression and critical for organized vessel formation, modifies the rate and magnitude of ES cells acquiring genotypic and phenotypic characteristics of mesodermal tissues. Hemangioblast, blood cell, cardiomyocyte, and vascular differentiation was impaired in EphB4-/-ES cells in conjunction with decreased expression of mesoderm-associated, but not neuroectoderm-associated, genes. Therefore, EphB4 modulates the response to mesoderm induction signals. These data add differentiation kinetics to the known effects of ephrin receptors on mammalian cell migration and adhesion. We propose that modifying sensitivity to differentiation cues is a further means for ephrin receptors to contribute to tissue patterning and organization.
AB - Differentiation of pluripotent embryonic stem (ES) cells is associated with expression of fate-specifying gene products. Coordinated development, however, must involve modifying factors that enable differentiation and growth to adjust in response to local microenvironmental determinants. We report here that the ephrin receptor, EphB4, known to be spatially restricted in expression and critical for organized vessel formation, modifies the rate and magnitude of ES cells acquiring genotypic and phenotypic characteristics of mesodermal tissues. Hemangioblast, blood cell, cardiomyocyte, and vascular differentiation was impaired in EphB4-/-ES cells in conjunction with decreased expression of mesoderm-associated, but not neuroectoderm-associated, genes. Therefore, EphB4 modulates the response to mesoderm induction signals. These data add differentiation kinetics to the known effects of ephrin receptors on mammalian cell migration and adhesion. We propose that modifying sensitivity to differentiation cues is a further means for ephrin receptors to contribute to tissue patterning and organization.
UR - http://www.scopus.com/inward/record.url?scp=0347597774&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347597774&partnerID=8YFLogxK
U2 - 10.1182/blood-2003-04-1063
DO - 10.1182/blood-2003-04-1063
M3 - Article
C2 - 12958066
AN - SCOPUS:0347597774
VL - 103
SP - 100
EP - 109
JO - Blood
JF - Blood
SN - 0006-4971
IS - 1
ER -