TY - JOUR
T1 - Eosinophilic intracytoplasmic globules in pulmonary adenocarcinomas
T2 - A histochemical, immunohistochemical, and ultrastructural study of six cases
AU - Scroggs, Mark W.
AU - Roggli, Victor L.
AU - Fraire, Armando E.
AU - Sanfilippo, Fred
PY - 1989
Y1 - 1989
N2 - Intracytoplasmic globules have been described in a variety of neoplastic and nonneoplastic conditions, but remain poorly defined. In a review of 100 consecutive cases of lung carcinomas, six cases of mucin-positive adenocarcinoma demonstrated eosinophilic intracytoplasmic globules that ranged in size from <1 to 20 μ in diameter. The globules were often located adjacent to areas of tumor necrosis, and occurred either singly or multiply within individual tumor cells. Globules were similar in morphologic appearance to Russell bodies in plasma cells or the eosinophilic globules in hepatocytes of patients with alpha-1-antitrypsin deficiency, but were morphologically distinct from intracytoplasmic mucin vacuoles. The globules were brightly positive with PAS stain with diastase, were brick red with Masson's trichrome stain, and showed variably positive staining with Mallory's phosphotungstic acid-hematoxylin and Ziehl-Nielson stains. Immunoperoxidase staining showed slight sataining of some globules with albumin, IgG, IgA, and alpha-1-antitrypsin. Ultrastructurally the globules had a homogeneous density and were often associated with profiles of rough endoplasmic reticulum. We suggest that these globules represent secretory glyco-protein accumulated in the cytoplasm of tumor cells in areas of tumor cell injury.
AB - Intracytoplasmic globules have been described in a variety of neoplastic and nonneoplastic conditions, but remain poorly defined. In a review of 100 consecutive cases of lung carcinomas, six cases of mucin-positive adenocarcinoma demonstrated eosinophilic intracytoplasmic globules that ranged in size from <1 to 20 μ in diameter. The globules were often located adjacent to areas of tumor necrosis, and occurred either singly or multiply within individual tumor cells. Globules were similar in morphologic appearance to Russell bodies in plasma cells or the eosinophilic globules in hepatocytes of patients with alpha-1-antitrypsin deficiency, but were morphologically distinct from intracytoplasmic mucin vacuoles. The globules were brightly positive with PAS stain with diastase, were brick red with Masson's trichrome stain, and showed variably positive staining with Mallory's phosphotungstic acid-hematoxylin and Ziehl-Nielson stains. Immunoperoxidase staining showed slight sataining of some globules with albumin, IgG, IgA, and alpha-1-antitrypsin. Ultrastructurally the globules had a homogeneous density and were often associated with profiles of rough endoplasmic reticulum. We suggest that these globules represent secretory glyco-protein accumulated in the cytoplasm of tumor cells in areas of tumor cell injury.
KW - adenocarcinoma of the lung
KW - electron microscopy
KW - eosinophilic intracytoplasmic globules
KW - immunohistochemistry
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U2 - 10.1016/0046-8177(89)90095-6
DO - 10.1016/0046-8177(89)90095-6
M3 - Article
C2 - 2476374
AN - SCOPUS:0024431265
VL - 20
SP - 845
EP - 849
JO - Human Pathology
JF - Human Pathology
SN - 0046-8177
IS - 9
ER -