Eosinophil transendothelial migration induced by cytokines: II. Potentiation of eosinophil transendothelial migration by eosinophil-active cytokines

Motohiro Ebisawa, Mark C. Liu, Toshimitsu Yamada, Masato Kato, Lawrence M. Lichtenstein, Bruce S. Bochner, Robert P. Schleimer

Research output: Contribution to journalArticlepeer-review

Abstract

Activation of HUVEC monolayers by IL-1β or TNF-α induces migration of eosinophils across the endothelial monolayer (i.e., transendothelial migration) in vitro. In the present study, we demonstrate that culture of freshly isolated eosinophils in the presence of IL-5 for 24 to 48-h before use in the assay dramatically potentiated CD18-dependent eosinophil transendothelial migration through unstimulated endothelial monolayers. Granulocyte macrophage (GM)-CSF induced eosinophil transendothelial migration but did not induce neutrophil transendothelial migration. When IL-1β- activated endothelial cells were used, GM-CSF, IL-3, or IL-5 caused only modest potentiation of eosinophil transendothelial migration. Since activated endothelial cells are known to produce GM-CSF, we hypothesized that endothelial-derived GM-CSF might play a role in the process of IL-1β- induced eosinophil transendothelial migration. IL-1β-activated endothelial monolayers grown on the permeable supports produced 0.3 ± 0.1 ng/ml of GM- CSF during a 4-h incubation and neutralizing Ab against GM-CSF inhibited eosinophil transendothelial migration by 48%, suggesting that endothelial- derived GM-CSF may participate in the response. Eosinophils purified from bronchoalveolar lavage 18 to 20 h after experimental Ag challenge in the lungs of allergic volunteers showed enhanced transendothelial migration, indicating that the cells may have undergone cytokine activation in vivo. Eosinophil-active cytokines may contribute to the preferential accumulation of eosinophils in vivo in part via potentiation of eosinophil transendothelial migration.

Original languageEnglish (US)
Pages (from-to)4590-4596
Number of pages7
JournalJournal of Immunology
Volume152
Issue number9
StatePublished - May 1 1994

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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