Enzyme-linked immunosorbent microassay and hemagglutinin compared for detection of thyroglobulin and thyroid microsomal autoantibodies

Sheila H. Roman, F. Korn, T. F. Davies

Research output: Contribution to journalArticle

Abstract

We have evaluated for their potential use in the routine clinical laboratory enzyme-linked immunosorbent assays (ELISA) for human thyroglobulin antibodies (hTg-Ab) and microsomal antibodies (M-Ab). Results are expressed in terms of an 'ELISA Index', based on comparison with a laboratory standard. The specificity of both ELISA assays is shown by dose-dependent inhibition on the hTg-Ab and M-Ab activities of the laboratory standards by the appropriate specific antigens. Similar concentrations of ovalbumin had no significant effect on the standard activity in both assays. For consecutive samples evaluated for hTg-Ab (n = 113) and M-Ab (n = 106) by ELISA and hemagglutinin, rank order analysis of the results showed a highly significant correlation between the methods (r = 0.81, p =

Original languageEnglish (US)
Pages (from-to)246-251
Number of pages6
JournalClinical Chemistry
Volume30
Issue number2
StatePublished - 1984
Externally publishedYes

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Immunosorbents
Thyroglobulin
Hemagglutinins
Autoantibodies
Assays
Thyroid Gland
Antibodies
Enzymes
Enzyme-Linked Immunosorbent Assay
Clinical laboratories
Ovalbumin
Antigens

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Enzyme-linked immunosorbent microassay and hemagglutinin compared for detection of thyroglobulin and thyroid microsomal autoantibodies. / Roman, Sheila H.; Korn, F.; Davies, T. F.

In: Clinical Chemistry, Vol. 30, No. 2, 1984, p. 246-251.

Research output: Contribution to journalArticle

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