Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin

R. H. Yolken, H. B. Greenberg, M. H. Merson, R. B. Sack, A. Z. Kapikian

Research output: Contribution to journalArticle

Abstract

The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.

Original languageEnglish (US)
Pages (from-to)439-444
Number of pages6
JournalJournal of clinical microbiology
Volume6
Issue number5
StatePublished - Dec 1 1977

ASJC Scopus subject areas

  • Microbiology (medical)

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    Yolken, R. H., Greenberg, H. B., Merson, M. H., Sack, R. B., & Kapikian, A. Z. (1977). Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. Journal of clinical microbiology, 6(5), 439-444.