Enzyme-linked immunosorbent assay for detection and identification of coxsackieviruses A

Robert H Yolken, V. M. Torsch

Research output: Contribution to journalArticle

Abstract

Coxsackieviruses A are known to cause a wide range of human disease processes. However, because many coxsackieviruses A present in clinical specimens do not produce a recognizable cytopathic effect in readily available tissue culture systems, infections with coxsackieviruses A are often difficult to diagnose. We have thus developed enzyme-linked immunosorbent assay (ELISA) systems for the detection and serotyping of coxsackievirus A antigens. The assays consist of a double-antibody ELISA which utilizes type-specific monkey and mouse coxsackievirus antisera. Although some cross-reactivity was noted, the ELISA systems correctly identified the serotypes of 22 of 23 coxsackievirus A complement fixation antigens available for testing. Testing of tissue culture fluids revealed that antigen could often be detected by ELISA before the appearance of a cytopathic effect. In addition, the infecting coxsackievirus A antigen could be unequivocally identified in 8 of 11 stool specimens obtained from patients with coxsackievirus A infections. The ELISA system might thus represent an important tool in the diagnosis and study of coxsackievirus A infections.

Original languageEnglish (US)
Pages (from-to)742-750
Number of pages9
JournalInfection and Immunity
Volume31
Issue number2
StatePublished - 1981

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Enterovirus
Enzyme-Linked Immunosorbent Assay
Coxsackievirus Infections
Antigens
Serotyping
Haplorhini
Immune Sera
Antibodies

ASJC Scopus subject areas

  • Immunology

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Enzyme-linked immunosorbent assay for detection and identification of coxsackieviruses A. / Yolken, Robert H; Torsch, V. M.

In: Infection and Immunity, Vol. 31, No. 2, 1981, p. 742-750.

Research output: Contribution to journalArticle

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