Enzyme-linked immunosorbent assay (ELISA): A practical tool for rapid diagnosis of viruses and other infectious agents

Research output: Contribution to journalArticlepeer-review

Abstract

Tissue culture techniques are inadequate to diagnose some viral infections. Thus, solid-phase immunoassays have been developed for the direct detection of viral antigens in clinical specimens. While radioimmunoassays (RIA) have attained widespread use, solid-phase enzyme-linked immunosorbent assays (ELISA) offer a number of advantages over RIA systems. ELISAs can be established with approximately the same sensitivity as radioimmunoassays without utilizing unstable, gamma-emitting isotopes. However, before ELISA systems can obtain widespread usage, a number of aspects of the test must be optimized. These include the preparation and use of reagents, the nature of the solid phase, the choice of enzyme, and the enzyme-antibody conjugation method. With the solving of these problems, ELISA should attain widespread usage for rapid diagnosis of a large number of infectious agents.

Original languageEnglish (US)
Pages (from-to)85-92
Number of pages8
JournalYale Journal of Biology and Medicine
Volume53
Issue number1
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Enzyme-linked immunosorbent assay (ELISA): A practical tool for rapid diagnosis of viruses and other infectious agents'. Together they form a unique fingerprint.

Cite this