Enzyme immunoassays for detection of Clostridium difficile toxins A and B in fecal specimens

B. E. Laughon, Raphael P Viscidi, S. L. Gdovin, Robert H Yolken, John Bartlett

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Abstract

Enzyme immunoassays for detection of Clostridium difficile toxins A and B were developed with use of a double-sandwich microtiter plate format. Each assay was specific for its respective toxin and was sensitive to 0.1 ng of toxin. Neither assay was reactive with 13 other species of clostridia. One hundred fifty fecal specimens submitted for tissue culture cytotoxicity assay were evaluated by enzyme-linked immunosorbent assay (ELISA). Of the 79 tissue culture-positive specimens, 72 (91%) were positive in the A assay, 63 (80%) were positive in the B assay, and 75 (95%) were positive in either assay. Specimens with tissue culture titers of α>103 were uniformly positive in both assays. The specificities of the toxin A and B ELISAs were 98.6% and 100% respectively. An ELISA for both toxins could serve as a substitute for the tissue culture cytotoxicity assay.

Original languageEnglish (US)
Pages (from-to)781-788
Number of pages8
JournalJournal of Infectious Diseases
Volume149
Issue number5
Publication statusPublished - 1984

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ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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