@article{aa9e81ac2020445ca58bb260913e06ef,
title = "Enzyme immunoassay for the detection of clostridium difficile antigen",
author = "Yolken, {R. H.} and Whitcomb, {L. S.} and G. Marien and Bartlett, {J. D.} and J. Libby and M. Ehrich and T. Wilkins",
note = "Funding Information: Goat and rabbit antisera to C. difficile were prepared as previously described [2]. The EIA was adapted from procedures that had been devised for the measurement of viruses and toxins in human stool [3]. The rabbit antiserum to C. difficile was used to coat the wells of polyvinyl microtitration plates, and the goat antiserum to C. difficile was used as the second antibody. The amount of goat antibody bound to the specimen that was added to the solid phase was measured by the subsequent addition of alkaline phosphatase-labeled antibody to goat IgG and p-nitrophenol phosphate substrate. Stool specimens were tested as 10010 suspensions in phosphate- This work was supported in part by funds from The Hospital for Consumptives of Maryland (Eudowood) and from the Thrasher ResearchFund and by grants no. NOI AI 92616 and I ROI AI 15749-01 from the National Institute of Allergyand Infectious Diseases. Please address requests for reprints to Dr. R. H. Yolken, Department of Pediatrics, The Johns Hopkins Hospital, Baltimore, Maryland 21205.",
year = "1981",
doi = "10.1093/infdis/144.4.378",
language = "English (US)",
volume = "144",
pages = "378",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "4",
}