Enzyme activities and maturation in unstimulated and exogenous gonadotropin-stimulated human oocytes

R. A. Yazigi, M. M.Y. Chi, D. S. Mastrogiannis, R. C. Strickler, V. C. Yang, O. H. Lowry

Research output: Contribution to journalArticle

Abstract

With the advent of new techniques of human in vitro fertilization (IVF), identifying parameters of oocyte quality to allow selection of those most likely to fertilize becomes crucial. Morphology of oocytes, which correlates positively with biological performance, is the currently utilized classification criterion. However, biological links between form and function are tenuous, and underlying mechanisms remain elusive. We investigated whether biochemical activation is quantitatively associated with the stages of maturation in ova obtained from patients undergoing gynecologic surgery during unstimulated cycles and women undergoing IVF after exogenous gonadotropin stimulation. Changes in selected enzymes from protein, lipid, and carbohydrate metabolism (hexokinase, phosphoglucomutase, glycogen synthetase, uridine diphosphoglucose pyrophosphorylase, glucose-6-phosphate dehydrogenase, cytosolic thiolase, β-hydroxyacyl-CoA dehydrogenase, alanine aminotransferase, and aspartate aminotransferase) were determined simultaneously, in individual oocytes, utilizing a highly sensitive biochemical methodology. Several enzyme activities paralleled maturation grade and were higher in stimulated oocytes after correction for grade. These biochemical findings quantify metabolic and functional changes that increase as ova mature, possibly contributing to their reproductive performance.

Original languageEnglish (US)
Pages (from-to)C951-C955
JournalAmerican Journal of Physiology - Cell Physiology
Volume264
Issue number4 33-4
DOIs
StatePublished - 1993

Keywords

  • correlation of metabolic enzymes
  • in vitro fertilization
  • reproduction

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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    Yazigi, R. A., Chi, M. M. Y., Mastrogiannis, D. S., Strickler, R. C., Yang, V. C., & Lowry, O. H. (1993). Enzyme activities and maturation in unstimulated and exogenous gonadotropin-stimulated human oocytes. American Journal of Physiology - Cell Physiology, 264(4 33-4), C951-C955. https://doi.org/10.1152/ajpcell.1993.264.4.c951