Enzymatically triggered actuation of miniaturized tools

Noy Bassik, Alla Brafman, Aasiyeh M. Zarafshar, Mustapha Jamal, Delgermaa Luvsanjav, Florin Selaru, David H. Gracias

Research output: Contribution to journalArticle

Abstract

We demonstrate a methodology that utilizes the specificity of enzyme-substrate biomolecular interactions to trigger miniaturized tools under biocompatible conditions. Miniaturized grippers were constructed using multilayer hinges that employed intrinsic strain energy and biopolymer triggers, as well as ferromagnetic elements. This composition obviated the need for external energy sources and allowed for remote manipulation of the tools. Selective enzymatic degradation of biopolymer hinge components triggered closing of the grippers; subsequent reopening was achieved with an orthogonal enzyme. We highlight the utility of these enzymatically triggered tools by demonstrating the biopsy of liver tissue from a model organ system and gripping and releasing an alginate bead. This strategy suggests an approach for the development of smart materials and devices that autonomously reconfigure in response to extremely specific biological environments.

Original languageEnglish (US)
Pages (from-to)16314-16317
Number of pages4
JournalJournal of the American Chemical Society
Volume132
Issue number46
DOIs
StatePublished - Nov 24 2010

Fingerprint

Biopolymers
Grippers
Hinges
Enzymes
Substrate Specificity
Intelligent materials
Biopsy
Alginate
Strain energy
Equipment and Supplies
Liver
Multilayers
Tissue
Degradation
Substrates
Chemical analysis
alginic acid

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Bassik, N., Brafman, A., Zarafshar, A. M., Jamal, M., Luvsanjav, D., Selaru, F., & Gracias, D. H. (2010). Enzymatically triggered actuation of miniaturized tools. Journal of the American Chemical Society, 132(46), 16314-16317. https://doi.org/10.1021/ja106218s

Enzymatically triggered actuation of miniaturized tools. / Bassik, Noy; Brafman, Alla; Zarafshar, Aasiyeh M.; Jamal, Mustapha; Luvsanjav, Delgermaa; Selaru, Florin; Gracias, David H.

In: Journal of the American Chemical Society, Vol. 132, No. 46, 24.11.2010, p. 16314-16317.

Research output: Contribution to journalArticle

Bassik, N, Brafman, A, Zarafshar, AM, Jamal, M, Luvsanjav, D, Selaru, F & Gracias, DH 2010, 'Enzymatically triggered actuation of miniaturized tools', Journal of the American Chemical Society, vol. 132, no. 46, pp. 16314-16317. https://doi.org/10.1021/ja106218s
Bassik N, Brafman A, Zarafshar AM, Jamal M, Luvsanjav D, Selaru F et al. Enzymatically triggered actuation of miniaturized tools. Journal of the American Chemical Society. 2010 Nov 24;132(46):16314-16317. https://doi.org/10.1021/ja106218s
Bassik, Noy ; Brafman, Alla ; Zarafshar, Aasiyeh M. ; Jamal, Mustapha ; Luvsanjav, Delgermaa ; Selaru, Florin ; Gracias, David H. / Enzymatically triggered actuation of miniaturized tools. In: Journal of the American Chemical Society. 2010 ; Vol. 132, No. 46. pp. 16314-16317.
@article{af3de5830bd24551be78031c79e6e8de,
title = "Enzymatically triggered actuation of miniaturized tools",
abstract = "We demonstrate a methodology that utilizes the specificity of enzyme-substrate biomolecular interactions to trigger miniaturized tools under biocompatible conditions. Miniaturized grippers were constructed using multilayer hinges that employed intrinsic strain energy and biopolymer triggers, as well as ferromagnetic elements. This composition obviated the need for external energy sources and allowed for remote manipulation of the tools. Selective enzymatic degradation of biopolymer hinge components triggered closing of the grippers; subsequent reopening was achieved with an orthogonal enzyme. We highlight the utility of these enzymatically triggered tools by demonstrating the biopsy of liver tissue from a model organ system and gripping and releasing an alginate bead. This strategy suggests an approach for the development of smart materials and devices that autonomously reconfigure in response to extremely specific biological environments.",
author = "Noy Bassik and Alla Brafman and Zarafshar, {Aasiyeh M.} and Mustapha Jamal and Delgermaa Luvsanjav and Florin Selaru and Gracias, {David H.}",
year = "2010",
month = "11",
day = "24",
doi = "10.1021/ja106218s",
language = "English (US)",
volume = "132",
pages = "16314--16317",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "46",

}

TY - JOUR

T1 - Enzymatically triggered actuation of miniaturized tools

AU - Bassik, Noy

AU - Brafman, Alla

AU - Zarafshar, Aasiyeh M.

AU - Jamal, Mustapha

AU - Luvsanjav, Delgermaa

AU - Selaru, Florin

AU - Gracias, David H.

PY - 2010/11/24

Y1 - 2010/11/24

N2 - We demonstrate a methodology that utilizes the specificity of enzyme-substrate biomolecular interactions to trigger miniaturized tools under biocompatible conditions. Miniaturized grippers were constructed using multilayer hinges that employed intrinsic strain energy and biopolymer triggers, as well as ferromagnetic elements. This composition obviated the need for external energy sources and allowed for remote manipulation of the tools. Selective enzymatic degradation of biopolymer hinge components triggered closing of the grippers; subsequent reopening was achieved with an orthogonal enzyme. We highlight the utility of these enzymatically triggered tools by demonstrating the biopsy of liver tissue from a model organ system and gripping and releasing an alginate bead. This strategy suggests an approach for the development of smart materials and devices that autonomously reconfigure in response to extremely specific biological environments.

AB - We demonstrate a methodology that utilizes the specificity of enzyme-substrate biomolecular interactions to trigger miniaturized tools under biocompatible conditions. Miniaturized grippers were constructed using multilayer hinges that employed intrinsic strain energy and biopolymer triggers, as well as ferromagnetic elements. This composition obviated the need for external energy sources and allowed for remote manipulation of the tools. Selective enzymatic degradation of biopolymer hinge components triggered closing of the grippers; subsequent reopening was achieved with an orthogonal enzyme. We highlight the utility of these enzymatically triggered tools by demonstrating the biopsy of liver tissue from a model organ system and gripping and releasing an alginate bead. This strategy suggests an approach for the development of smart materials and devices that autonomously reconfigure in response to extremely specific biological environments.

UR - http://www.scopus.com/inward/record.url?scp=78649909612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649909612&partnerID=8YFLogxK

U2 - 10.1021/ja106218s

DO - 10.1021/ja106218s

M3 - Article

C2 - 20849106

AN - SCOPUS:78649909612

VL - 132

SP - 16314

EP - 16317

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 46

ER -