Enzymatic incorporation of multiple dyes for increased sensitivity in QD-FRET sensing for DNA methylation detection

Vasudev J. Bailey, Brian P. Keeley, Yi Zhang, Yi Ping Ho, Hariharan Easwaran, Malcolm V. Brock, Kristen L. Pelosky, Hetty E. Carraway, Stephen B. Baylin, James G. Herman, Tza Huei Wang

Research output: Contribution to journalArticlepeer-review

Abstract

(Chemical Equation Presented) Come closer to the light: Fluorescently labeled cytosines have been enzymatically incorporated within bisulfite-treated DNA that was amplified by using methylation-specific primers. Detection of these multilabeled products was carried out by using quantum-dot FRET (QD-FRET).

Original languageEnglish (US)
Pages (from-to)71-74
Number of pages4
JournalChemBioChem
Volume11
Issue number1
DOIs
StatePublished - Jan 4 2010

Keywords

  • Biosensors
  • DNA
  • Methylation
  • Optical analysis
  • Spectroscopic methods

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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