A novel method for the sensitive and specific microestimation of steroids has been described. This method depends upon the selective oxidation or reduction of respectively hydroxy- and ketosteroids by highly purified hydroxysteroid dehydrogenases of bacterial origin. The method permits the estimation of 3a-hydroxysteroids, 3β-(and 17β-) hydroxysteroids, 3-ketosteroids and 17-ketosteroids.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the Society for Experimental Biology and Medicine|
|State||Published - Dec 1956|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)