Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1

Elena Ferrari, Amy Tsay, Peyton A. Eggleston, Alberto Spisni, Martin D. Chapman

Research output: Contribution to journalArticle

Abstract

Background Mouse urinary allergens are an important cause of occupational asthma in animal facilities. Domestic exposure to mouse allergens is a risk factor for asthma among inner-city residents. Objective We sought to develop a sensitive and specific assay for assessing environmental mouse allergen exposure. Methods An ELISA for recombinant (r)Mus m 1 was developed by using rabbit polyclonal antibodies to rMus m 1 that were affinity purified against the natural allergen. Assay specificity was established by means of immunoblotting and ELISA. Mus m 1 levels in mouse, other mammalian allergenic products, and house dust samples from inner-city homes were compared. Results Polyclonal antibodies to Mus m 1 showed a single 20-kd band on immunoblots against rMus m 1 and male mouse urine. Parallel dose-response curves were obtained by using mouse urine extract and natural Mus m 1 or rMus m 1. Mus m 1 was detected in mouse allergenic products (0.10-10.0 μg/mL) and in gerbil allergenic products (0.1 μg/mL) but was less than the limit of detection in epithelial extracts from 10 other animal species. Environmental measurements showed an excellent correlation between Mus m 1 levels in house dust extracts from inner-city asthma studies by using 2 different Mus m 1 standards (n=22; r=0.99; P <.001). Conclusions A highly sensitive ELISA has been developed with rMus m 1. This assay is suitable for monitoring domestic and environmental exposure to mouse urinary allergens.

Original languageEnglish (US)
Pages (from-to)341-346
Number of pages6
JournalThe Journal of Allergy and Clinical Immunology
Volume114
Issue number2
DOIs
StatePublished - Aug 2004

Fingerprint

Immunoassay
Allergens
Enzyme-Linked Immunosorbent Assay
Dust
Asthma
Urine
Occupational Asthma
Gerbillinae
Antibodies
Environmental Exposure
Immunoblotting
Limit of Detection

Keywords

  • allergen standardization
  • immunoassay
  • in vitro diagnostics
  • inner-city asthma
  • Mouse allergen
  • Mouse urinary protein
  • MUP
  • occupational asthma
  • PBS, pH 7.4 containing 0.05% Tween 20
  • PBS-T
  • recombinant allergen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1. / Ferrari, Elena; Tsay, Amy; Eggleston, Peyton A.; Spisni, Alberto; Chapman, Martin D.

In: The Journal of Allergy and Clinical Immunology, Vol. 114, No. 2, 08.2004, p. 341-346.

Research output: Contribution to journalArticle

Ferrari, Elena ; Tsay, Amy ; Eggleston, Peyton A. ; Spisni, Alberto ; Chapman, Martin D. / Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1. In: The Journal of Allergy and Clinical Immunology. 2004 ; Vol. 114, No. 2. pp. 341-346.
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