Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1

Elena Ferrari; Amy Tsay; Peyton A. Eggleston; Alberto Spisni; Martin D. Chapman

doi:10.1016/j.jaci.2004.04.028

Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1

Elena Ferrari, Amy Tsay, Peyton A. Eggleston, Alberto Spisni, Martin D. Chapman

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Background Mouse urinary allergens are an important cause of occupational asthma in animal facilities. Domestic exposure to mouse allergens is a risk factor for asthma among inner-city residents. Objective We sought to develop a sensitive and specific assay for assessing environmental mouse allergen exposure. Methods An ELISA for recombinant (r)Mus m 1 was developed by using rabbit polyclonal antibodies to rMus m 1 that were affinity purified against the natural allergen. Assay specificity was established by means of immunoblotting and ELISA. Mus m 1 levels in mouse, other mammalian allergenic products, and house dust samples from inner-city homes were compared. Results Polyclonal antibodies to Mus m 1 showed a single 20-kd band on immunoblots against rMus m 1 and male mouse urine. Parallel dose-response curves were obtained by using mouse urine extract and natural Mus m 1 or rMus m 1. Mus m 1 was detected in mouse allergenic products (0.10-10.0 μg/mL) and in gerbil allergenic products (0.1 μg/mL) but was less than the limit of detection in epithelial extracts from 10 other animal species. Environmental measurements showed an excellent correlation between Mus m 1 levels in house dust extracts from inner-city asthma studies by using 2 different Mus m 1 standards (n=22; r=0.99; P < .001). Conclusions A highly sensitive ELISA has been developed with rMus m 1. This assay is suitable for monitoring domestic and environmental exposure to mouse urinary allergens.

Original language	English (US)
Pages (from-to)	341-346
Number of pages	6
Journal	Journal of Allergy and Clinical Immunology
Volume	114
Issue number	2
DOIs	https://doi.org/10.1016/j.jaci.2004.04.028
State	Published - Aug 2004
Externally published	Yes

Keywords

MUP
Mouse allergen
Mouse urinary protein
PBS, pH 7.4 containing 0.05% Tween 20
PBS-T
allergen standardization
immunoassay
in vitro diagnostics
inner-city asthma
occupational asthma
recombinant allergen

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.jaci.2004.04.028

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@article{59f78e3637d44e3588ff1de5cd6c3f26,

title = "Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1",

abstract = "Background Mouse urinary allergens are an important cause of occupational asthma in animal facilities. Domestic exposure to mouse allergens is a risk factor for asthma among inner-city residents. Objective We sought to develop a sensitive and specific assay for assessing environmental mouse allergen exposure. Methods An ELISA for recombinant (r)Mus m 1 was developed by using rabbit polyclonal antibodies to rMus m 1 that were affinity purified against the natural allergen. Assay specificity was established by means of immunoblotting and ELISA. Mus m 1 levels in mouse, other mammalian allergenic products, and house dust samples from inner-city homes were compared. Results Polyclonal antibodies to Mus m 1 showed a single 20-kd band on immunoblots against rMus m 1 and male mouse urine. Parallel dose-response curves were obtained by using mouse urine extract and natural Mus m 1 or rMus m 1. Mus m 1 was detected in mouse allergenic products (0.10-10.0 μg/mL) and in gerbil allergenic products (0.1 μg/mL) but was less than the limit of detection in epithelial extracts from 10 other animal species. Environmental measurements showed an excellent correlation between Mus m 1 levels in house dust extracts from inner-city asthma studies by using 2 different Mus m 1 standards (n=22; r=0.99; P < .001). Conclusions A highly sensitive ELISA has been developed with rMus m 1. This assay is suitable for monitoring domestic and environmental exposure to mouse urinary allergens.",

keywords = "MUP, Mouse allergen, Mouse urinary protein, PBS, pH 7.4 containing 0.05% Tween 20, PBS-T, allergen standardization, immunoassay, in vitro diagnostics, inner-city asthma, occupational asthma, recombinant allergen",

author = "Elena Ferrari and Amy Tsay and Eggleston, {Peyton A.} and Alberto Spisni and Chapman, {Martin D.}",

note = "Funding Information: Supported in part by a cofinancing research grant to AS from the Ministero Industria Universita e Ricerca, Italy (COFIN MURST). ",

year = "2004",

month = aug,

doi = "10.1016/j.jaci.2004.04.028",

language = "English (US)",

volume = "114",

pages = "341--346",

journal = "Journal of Allergy and Clinical Immunology",

issn = "0091-6749",

publisher = "Mosby Inc.",

number = "2",

}

TY - JOUR

T1 - Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1

AU - Ferrari, Elena

AU - Tsay, Amy

AU - Eggleston, Peyton A.

AU - Spisni, Alberto

AU - Chapman, Martin D.

N1 - Funding Information: Supported in part by a cofinancing research grant to AS from the Ministero Industria Universita e Ricerca, Italy (COFIN MURST).

PY - 2004/8

Y1 - 2004/8

N2 - Background Mouse urinary allergens are an important cause of occupational asthma in animal facilities. Domestic exposure to mouse allergens is a risk factor for asthma among inner-city residents. Objective We sought to develop a sensitive and specific assay for assessing environmental mouse allergen exposure. Methods An ELISA for recombinant (r)Mus m 1 was developed by using rabbit polyclonal antibodies to rMus m 1 that were affinity purified against the natural allergen. Assay specificity was established by means of immunoblotting and ELISA. Mus m 1 levels in mouse, other mammalian allergenic products, and house dust samples from inner-city homes were compared. Results Polyclonal antibodies to Mus m 1 showed a single 20-kd band on immunoblots against rMus m 1 and male mouse urine. Parallel dose-response curves were obtained by using mouse urine extract and natural Mus m 1 or rMus m 1. Mus m 1 was detected in mouse allergenic products (0.10-10.0 μg/mL) and in gerbil allergenic products (0.1 μg/mL) but was less than the limit of detection in epithelial extracts from 10 other animal species. Environmental measurements showed an excellent correlation between Mus m 1 levels in house dust extracts from inner-city asthma studies by using 2 different Mus m 1 standards (n=22; r=0.99; P < .001). Conclusions A highly sensitive ELISA has been developed with rMus m 1. This assay is suitable for monitoring domestic and environmental exposure to mouse urinary allergens.

AB - Background Mouse urinary allergens are an important cause of occupational asthma in animal facilities. Domestic exposure to mouse allergens is a risk factor for asthma among inner-city residents. Objective We sought to develop a sensitive and specific assay for assessing environmental mouse allergen exposure. Methods An ELISA for recombinant (r)Mus m 1 was developed by using rabbit polyclonal antibodies to rMus m 1 that were affinity purified against the natural allergen. Assay specificity was established by means of immunoblotting and ELISA. Mus m 1 levels in mouse, other mammalian allergenic products, and house dust samples from inner-city homes were compared. Results Polyclonal antibodies to Mus m 1 showed a single 20-kd band on immunoblots against rMus m 1 and male mouse urine. Parallel dose-response curves were obtained by using mouse urine extract and natural Mus m 1 or rMus m 1. Mus m 1 was detected in mouse allergenic products (0.10-10.0 μg/mL) and in gerbil allergenic products (0.1 μg/mL) but was less than the limit of detection in epithelial extracts from 10 other animal species. Environmental measurements showed an excellent correlation between Mus m 1 levels in house dust extracts from inner-city asthma studies by using 2 different Mus m 1 standards (n=22; r=0.99; P < .001). Conclusions A highly sensitive ELISA has been developed with rMus m 1. This assay is suitable for monitoring domestic and environmental exposure to mouse urinary allergens.

KW - MUP

KW - Mouse allergen

KW - Mouse urinary protein

KW - PBS, pH 7.4 containing 0.05% Tween 20

KW - PBS-T

KW - allergen standardization

KW - immunoassay

KW - in vitro diagnostics

KW - inner-city asthma

KW - occupational asthma

KW - recombinant allergen

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M3 - Article

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AN - SCOPUS:4344630833

SN - 0091-6749

VL - 114

SP - 341

EP - 346

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

IS - 2

ER -

Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1

Abstract

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