Environmental and genetic determinants of aflatoxin-albumin adducts in The Gambia

Christopher P. Wild, Fen Yin, Paul C. Turner, Isabelle Chemin, Brigitte Chapot, Maimuna Mendy, Hilton Whittle, Gregory D. Kirk, Andrew J. Hall

Research output: Contribution to journalArticlepeer-review

Abstract

Aflatoxins together with chronic hepatitis B virus (HBV) infection contribute to the high incidence of hepatocellular carcinoma in developing countries. An understanding of the mechanism of interaction between these factors would provide a strong rationale for developing effective prevention strategies. In this study in The Gambia we examined the effect of environmental (place of residence and timing of sample collection) and host factors (age, sex, HBV status and interindividual variations in carcinogen metabolising enzymes) in determining blood aflatoxin-albumin adduct levels in 357 individuals of whom 181 were chronic HBV carriers. Samples were analysed for aflatoxin-albumin adducts, HBV status and genotypes of glutathione S- transferase (GST) MI, GSTTI, GSTPI and epoxide hydrolase (EPXH). Urine samples were analysed for 6β-hydroxycortisol:cortisol ratio as a marker of cytochrome P450 (CYP) 3A4 activity. Adduct levels were significantly higher in subjects resident in rural [geometric mean adduct level 34.9 pg aflatoxin B1-lysine equivalent (28.5-42.8; 95% CI)/mg albumin] than in periurban areas [22.2 pg (14.9-33.4)/mg] and were approximately twice as high in the dry season [mid-February to March; 83.2 pg (53.3130.8)/mg] than the wet [July to August; 34.9 pg (28.5-42.8)/mg]. In contrast, MBV status, CYP3A4 phenotype, GSTTI, GSTPI and EPXH genotypes were not associated with aflatoxin-albumin adduct level. However, mean adduct levels were significantly higher in non- HBV infected subjects with GSTMI null genotype. The main factors which affect aflatoxin-albumin adduct levels in this population are environmental, notably place of residence and timing of sample collection. This study further emphasises the priority to reduce aflatoxin exposure in these communities by primary prevention measures. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalInternational Journal of Cancer
Volume86
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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