Envelope vaccination shapes viral envelope evolution following simian immunodeficiency virus infection in rhesus monkeys

Aravind Basavapathruni, Wendy W. Yeh, Rory T. Coffey, James B. Whitney, Peter T. Hraber, Ayush Giri, Bette T. Korber, Srinivas S. Rao, Gary J. Nabel, John R. Mascola, Michael S. Seaman, Norman L. Letvin

Research output: Contribution to journalArticle

Abstract

The evolution of envelope mutations by replicating primate immunodeficiency viruses allows these viruses to escape from the immune pressure mediated by neutralizing antibodies. Vaccine-induced anti-envelope antibody responses may accelerate and/or alter the specificity of the antibodies, thus shaping the evolution of envelope mutations in the replicating virus. To explore this possibility, we studied the neutralizing antibody response and the envelope sequences in rhesus monkeys vaccinated with either gag-pol-nef immunogens or gag-pol-nef immunogens in combination with env and then infected with simian immunodeficiency virus (SIV). Using a pseudovirion neutralization assay, we demonstrate that envelope vaccination primed for an accelerated neutralizing antibody response following virus challenge. To monitor viral envelope evolution in these two cohorts of monkeys, full-length envelopes from plasma virus isolated at weeks 37 and 62 postchallenge were sequenced by single genome amplification to identify sites of envelope mutations. We show that env vaccination was associated with a change in the pattern of envelope mutations. Prevalent mutations in sequences from gag-pol-nef vaccinees included deletions in both variable regions 1 and 4 (V1 and V4), whereas deletions in the env vaccinees occurred only in V1. These data show that env vaccination altered the focus of the antibodymediated selection pressure on the evolution of envelope following SIV challenge.

Original languageEnglish (US)
Pages (from-to)953-963
Number of pages11
JournalJournal of Virology
Volume84
Issue number2
DOIs
StatePublished - Jan 2010
Externally publishedYes

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Simian immunodeficiency virus
Simian Immunodeficiency Virus
Virus Diseases
Macaca mulatta
Vaccination
vaccination
mutation
viruses
Mutation
Neutralizing Antibodies
neutralizing antibodies
Viruses
Antibody Formation
infection
Primate Lentiviruses
antigens
Pressure
Antibody Specificity
immunosuppression
neutralization tests

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Basavapathruni, A., Yeh, W. W., Coffey, R. T., Whitney, J. B., Hraber, P. T., Giri, A., ... Letvin, N. L. (2010). Envelope vaccination shapes viral envelope evolution following simian immunodeficiency virus infection in rhesus monkeys. Journal of Virology, 84(2), 953-963. https://doi.org/10.1128/JVI.01679-09

Envelope vaccination shapes viral envelope evolution following simian immunodeficiency virus infection in rhesus monkeys. / Basavapathruni, Aravind; Yeh, Wendy W.; Coffey, Rory T.; Whitney, James B.; Hraber, Peter T.; Giri, Ayush; Korber, Bette T.; Rao, Srinivas S.; Nabel, Gary J.; Mascola, John R.; Seaman, Michael S.; Letvin, Norman L.

In: Journal of Virology, Vol. 84, No. 2, 01.2010, p. 953-963.

Research output: Contribution to journalArticle

Basavapathruni, A, Yeh, WW, Coffey, RT, Whitney, JB, Hraber, PT, Giri, A, Korber, BT, Rao, SS, Nabel, GJ, Mascola, JR, Seaman, MS & Letvin, NL 2010, 'Envelope vaccination shapes viral envelope evolution following simian immunodeficiency virus infection in rhesus monkeys', Journal of Virology, vol. 84, no. 2, pp. 953-963. https://doi.org/10.1128/JVI.01679-09
Basavapathruni, Aravind ; Yeh, Wendy W. ; Coffey, Rory T. ; Whitney, James B. ; Hraber, Peter T. ; Giri, Ayush ; Korber, Bette T. ; Rao, Srinivas S. ; Nabel, Gary J. ; Mascola, John R. ; Seaman, Michael S. ; Letvin, Norman L. / Envelope vaccination shapes viral envelope evolution following simian immunodeficiency virus infection in rhesus monkeys. In: Journal of Virology. 2010 ; Vol. 84, No. 2. pp. 953-963.
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