Entropic exclusion by neurofilament sidearms: A mechanism for maintaining interfilament spacing

Henry G. Brown, Jan H. Hoh

Research output: Contribution to journalArticlepeer-review

Abstract

A long-range repulsive force near isolated neurofilaments was detected by exclusion of large molecules and by direct force measurements with atomic force microscopy. Adsorption of isolated native neurofilaments to a solid substrate in a high-salt solution (170 mM NaCl), in the presence of coisolating contaminants, shows that the contaminants are excluded from a zone that extends 50-100 nm from the core of the filament. Force-distance measurements by AFM show the presence of a weak repulsive force that extends >50 nm from the core of the filament; this repulsive force is absent in homopolymers of neurofilament L or trypsinized native filaments that lack the long sidearms present in native filaments. These results suggest that neurofilament sidearms form an entropic brush, thereby providing a mechanism for maintaining interfilament spacing.

Original languageEnglish (US)
Pages (from-to)15035-15040
Number of pages6
JournalBiochemistry
Volume36
Issue number49
DOIs
StatePublished - Dec 9 1997

ASJC Scopus subject areas

  • Biochemistry

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