Enrichment of human heterokaryons by ficoll gradient for complementation analysis of iduronate sulfatase deficiency

Lorraine R. Eisenberg, Barbara R. Migeon

Research output: Contribution to journalArticlepeer-review

Abstract

Ficoll gradients have been used to enrich for heterokaryons in cultures of human skin fibroblasts following polyethylene glycol (PEG) induced fusion. These gradients provide a simple and consistent method for obtaining populations of multinucleated cells, at least twofold greater than those resulting from fusion alone. Formation of glucose-6-phosphate dehydrogenase (G6PD) heteropolymers has been used as a functional assay for the presence of heterokaryons. Analysis of cell populations enriched for multinucleated cells has revealed complementation leading to iduronate sulfatase activity in heterokaryons derived from iduronate sulfatase-deficient fibroblasts expressing the Hunter and multiple sulfatase-deficiency mutations.

Original languageEnglish (US)
Pages (from-to)1079-1089
Number of pages11
JournalSomatic Cell Genetics
Volume5
Issue number6
DOIs
StatePublished - Nov 1 1979

ASJC Scopus subject areas

  • Genetics

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